1914} Actio x\ of \^akious Diuretics 41 



response of animals of different ages to the same quantity of 

 uranium are at present undetermined. 



THE EFFECT OF GREHANt's ANESTHETIC^ ON" THE OUTPUT AND 



COMPOSITION OF THE URINE IN YOUNG 



AND IN ADULT ANIMALS 



In the experiments that have been previously reported (1) in 

 which this anesthetic was employed, the anesthetic was used in 

 the full strength advised by its originator. In order to exclude 

 the possibility of the anesthetic unduly depressing the circula- 

 tion, in the present experiments the quantity of the anesthetic 

 has been reduced to 60 per cent strength. In this strength the 

 anesthetic was given to ten adult animals and eight young ani- 

 mals after they had been rendered nephritic by uranium. 



The ten adult animals following the development of an anes- 

 thesia which was not profound became anuric and remained 

 anuric throughout the experiments. The anuria was uninflu- 

 enced by the various diuretic substances which have been pre- 

 viously enumerated. 



The rapidity with which the anuria develops has varied slight- 

 ly in the different animals and is apparently dependent to some 

 extent upon the depth of the anesthesia. 



The following experiments illustrate this point: 

 Experiment 4. The animal was receiving 500 cc. of water daily. On the 

 day of the experiment the animal had an output of urine of 1130 cc. 

 Within forty minutes after the commencement of the anesthetic, which 

 resulted in a complete anesthesia, the animal became anuric. During the 

 course of the experiment no urine was obtained. At the commencement 

 of the experiment the animal had a blood pressure of 95 mm. of mercury, 

 and at its termination a blood pressure of 115 mm. of mercury. 



Following the injection of 1 cc. of a 1 per cent solution of caffeine per 

 kilogram, the general blood pressure was raised from 105 to 115 mm. of 

 mercury and the oncometer pressure as indicated by a water manometer, 

 showed a rise of 16 mm. of water. 



Experiment 7. In the following experiment the animal was imperfectly 

 anesthetized. During the operation he was constantly struggling. On the 

 day of the experiment the output of urine was 1025 cc. For the first 



' Grebanf s Anesthetic : The animal is given 0.25 cc. per kilogram of a 4 per 

 cent, solution of moi'phine. This is followed in half an hour by lOcc. per kilo- 

 gram of the following mixture : chloroform, 50cc. ; alcohol and water, each 

 500cc. 



