84 



Journal of the Mitchell Society [September 



southeastern Atlantic coast on the evening of September 2, 1913. 

 Only a few cultures were recovered and these somewhat mutilated. 

 As they show nothing that is not shown in the more complete series, 

 a report here is not deemed worth while. 



Considering now only those cultures which were successful and 

 omitting such as failed entirely because such failure was in all proba- 

 bility due to the conditions of the experiment, the results from Tables 

 1, 4, and 8 mav be summarized as follows : 



The writer thinks that the tetrasporic plants already discussed can 

 now safely be disregarded, and that there is nothing in the entire 

 series which in any way weakens the statement based upon the 1912 

 cultures — that tetraspores produce only male and female plants, and 

 these in equal numbers, even when the spores are from a single parent 

 plant, as well as the seemingly necessary deduction that sex is pre- 

 determined, probably in the reduction division of the tetraspore 

 mother cell. 



fertilized EGC4S 



The experiments to test the product of fertilized eggs as first de- 

 vised were baffling in the extreme (see Tables 2 and 5). These tables 

 are labeled '^Cultures derived from Male and Female Plants" because 

 it is believed, as will appear in the discussion to follow, that little or 

 no fertilization occurred. The shells and plants were treated as in 

 the experiments with tetrasporic plants. Four series of 4 shells each 

 were carried through in 1912. For ieach series one male and one 



