WALTER E. GARREY 



153 



following percentages of the normal rate; i.e., 311 per cent, 218 per 

 cent, 237 per cent, 145 per cent, 260 per cent, 134 per cent. Subse- 

 quent to these determinations immersion of the ganglia in the sodium- 

 calcium solution demonstrated the antagonism of these ions in their 

 effects on animal oxidations for the mixture caused a return of the 

 rate of respiration to within 15 per cent of the previous normal in 

 every instance. This fact excludes the possibility that the increase 

 in CO2 formation in the isotonic NaCl is a lethal phenomenon, in the 

 ordinary sense of that term, or at least that the stages dealt with 



TABLE III. 

 Effects of 1 per cent Ethyl Alcohol on Rate of CO2 Production. 



Condition of ganglion. 



In Na/Ca solution (normal) 



In Na/Ca + 1 per cent ethyl alcohol. 



In Na/Ca solution. 



In Na/Ca solution + 1 per cent alcohol 



Average 

 rate of COi 

 production. 



per cent 



100 



190 



103 



154 



are still reversible (Osterhout)^", Loeb and Wasteneys^^ working 

 with concentration of sodium chloride isotonic with sea water found a 

 decrease in oxidation in eggs of Arbacia and of Gofiione?nus; this 

 apparent disharmony with the behavior of Limulus heart ganglia 

 may be specific for the material used or may be merely an expression 

 of the duration of exposure to NaCl or of the concentrations of the 

 sodium chloride to which the tissue is subjected since Brooks'^ has 



JoOsterhout, W. J. V., /. Gen. Physiol., 1920-21, iii, 15. 



" Loeb, J., and Wasteneys, H., Biochem. Z., 1910, xxviii, 340; 1911, .\xxi, 168. 



" Brooks, M. M., /. Gen. Physiol., 1919-20, ii, 5. 



