236 



INACTIVATION OF TRYPSIN. I 



have been multiplied by the relative concentration of trypsin used in 

 obtaining the curve corresponding to that concentration. It will be 

 seen that if the formula QT = iv is correct, the resulting curves should 

 be identical (or parallel if plotted as in the figure). As the figure 

 shows this is not quite true. The curves for the concentrated solu- 

 tions do not drop ofi" as rapidly as those for the dilute. That is the 



0.6 0.8 1.0 



Time in nours 



Fig. 4. Comparison of the retardation due to the presence of the "inhibitor" 

 as determined by the formol titration or by the conductivity method, 



velocity as measured by the reciprocal of the time increases more 

 rapidly than the trypsin concentration. This is due, as will be shown 

 later, to two causes. First, the products formed by the action of the 

 trypsin inhibit its action, and since at any given stage of the hydroly- 

 sis the concentration of the products in the different solutions will 

 be the same but the concentration of trypsin different, the percentage 



