JOHN H. NORTKROP 



249 



TABLE IV. 

 EJJect of Increasing Amounts of Inhibitor on the Rate of Hydrolysis. 

 25 cc. gelatin pH 6.2; specific conductivity 2 X 10~^. 1 cc. trypsin (10 per 

 cent dialyzed). F = 28 cc. P = 10. K' = 2.8. 



18 



20 



4 6 8 10 12 14 16 



Units of inhibitop added (cc. inhibitor x lOj 



Fig. 1. Effect of adding increasing amounts of inhibitor to trypsin solutions 

 of different strengths. The solid curves are the calculated values, and the points, 

 the observed units of active trypsin present. (This is taken as the reciprocal 

 of the time in hours necessary to cause a change in the bridge reading of 10 

 points.) 



