PAUL H. DEKRUIF 



401 



was of 1 per cent concentration in distilled water. 

 recorded in Tables IV and V. 



The results are 



TABLE V. 

 Peptone Agglutination of Microbe D at Varying Ch+. 



20 



15 

 13 



7.5 7.0 6.5 6.0 55 5.0 4.5 4.0 3.5 aO 25 2.0 1.5 

 Fig. 3. 



It will be observed from Tables IV and V that the results are 

 strikingly different from those obtained with beef infusion. Peptone, 

 contrary to beef infusion, appears to shift the optimum zone of agglu- 

 tination in the direction of a higher Ch+, and effect analogous to tliat 

 observed in the glycocoll-HCl buffer mixtures (Table I). 



The optimum for Type G in the lactate and acetate buffer mi.xtures 

 lies between pH 5.0 and pH 3.8. Peptone changes the optimum to 

 pH 3.0 to 2.5. For Type D, an analogous effect is observed, the 



