JOHN H. NORTHROP 



501 



TABLE VII. 



Effect of Increasing the Concentration of Inhibitor. 2 Per Cent Gelatin. 



* Calculated from C = 



S {E - I cc.) 



; i.e., enzyme-inhibitor compound very 



K + S 



slightly dissociated; velocity proportional to combined enzyme (C). E = 2.9, 

 S = 2yK = 0.5, / = 4.0 units inhibitor per cc. of solution. 



t Calculated from Q = ■%/( " "^ ? ~ "^ \ +K E 



Vpl^ 



E 



1 ) 2 



Assumptions: (1) enzyme-inhibitor compound widely dissociated. (2) Rate of 

 hydrolysis proportional to /ree enzyme (0. K = 2.8, E = 2.3, d = cc. inhibiting 

 solution X 10. 



TABLE Vni. 

 Effect of Varying Enzyme Concentration with Constant Gelatin and Constant Inhibitor 



Concentrations. 

 Gelatin 2 per cent. 25 cc. + 1 cc. inhibiting solution + noted cc. trypsin 

 solution. 



* Calculated by formula C = 



S (El -I) 



,5= 2, / = 3.7, A' = 0.5, El as in 



K + S 



table. Assumptions: (1) enzyme-inhibitor compound slightly dissociated; 

 (2) velocity proportional to combined enzyme. 



t Calculated by same formula as (II), Table VII. d = 5.0. £2 as in table 

 above. 



