534 KINETICS OF BIOLUMINESCENT REACTION. I 



therefore, remains the unavoidable errors inherent in any photo- 

 graphic material. 



The optical pyrometer is a standard physical instrument and has 

 a considerable literature. Readers interested in fuller discussions of 

 its theory and practice should consult the papers of Forsythe.^^ 



EXPLANATION OF PLATE 1. 



Reproductions of actual photographic records. The bands are the moving 

 records; the fifteen spots the calibration exposures. 



(a) Effect of temperature. Temperatures, 16.5°C. and 24°C. 



(b) Effect of luciferin concentration, the low concentration having been obtained 

 by a brief heating of that solution to 55°C. 



C 



(c) Effect of luciferase concentrations, C and -• 



16 Hyde, E. P., Cady, F. E., and Forsythe, W. E., Astrophys. J., 1915, xlii, 

 294; Forsythe, W. E., 1919, xlix, 237; Am. Inst. Min. and Met. Eng., Bull. No. 

 153, 1919, 2547; /. Op. Soc. Am.,1920, iv, 305. 



