546 



KINETICS OF BIOLUMINESCENT REACTION. II 



therefore, represents the effect of the dilution upon the reaction 

 velocity. In this case this ratio is 2.17, a slightly higher value than 

 would be expected if reaction velocity were directly proportional to 

 enzyme concentration. Such a variation might well arise from the 

 experimental errors, but in the light of the data tabulated in Table 



OjO 



lj8 



O 

 1-1 _ 



ISi 



as 



2.4 



40 SO 120 



Time 



Fig. 4. Effect of enzyme concentration. Abscissae represent time in mm. along 



the film; ordinates, logarithm of intensity. 



kiovA (C) = 1.500 



k for B (A = 0.690 



Ratio = 2.17 



III, it will be seen that the slope ratios are practically always (one 

 exception) somewhat higher than the expectation for a direct propor- 

 tionality. It can be stated that the velocity of reaction is very nearly 

 proportional to enzyme concentration. 



