WILLIAM R. AMBERSON 



549 



tration of the oxyluciferin had been reduced by dilution to the proper 



C C C 



value. I then prepared C and — , C and — , and C and — luciferin 



solutions. 



The study of such solutions gave erratic and somewhat curious 

 results. In each separate curve the logarithmic relationship appeared 

 quite definitely, but the expected paralleHsm in the straight line 

 plottings did not develop. Instead, as Fig, 5 shows, the more dilute 



O 



40 



80 



120 



leo 



Time 

 Fig. 5. Effect of dilution of luciferin solutions. Abscissae represent time in 

 mm. along the film; ordinates, logarithm of intensity. 



klox A (C) = 1.080 



Mor5(^) = 



Ratio = 2.23 



0.484 



solution appeared, in all but one case, to fall through the decay curve 

 with a faster reaction velocity than its companion concentrated 

 solution. Temperature was carefully controlled, and enzyme strengths 

 were of course identical. I have tabulated some of these erratic results 



