WILLIAM R. AMBERSON 



553 



the logarithmic difference in the values of the two 3'-intercepts is 

 0.750, the initial brightness of solution B must have been 17.8 per 

 cent of solution A, and the luciferin concentration must be repre- 

 sented by the same figure. 



It follows that the total amount of light emitted from two solu- 

 tions differing in luciferin concentration will be in direct proportion 

 to those concentrations, for at every instant the light emitted by 

 the one will be the same constant fraction of the light emitted by the 

 other. I have previously used this relationship to determine indi- 

 rectly the form of the reaction in the secondary or spontaneous 

 reaction. 



6. The Temperature Coefficient. 



I have made a brief preliminary study of the temperature coefiScient, 

 and upon the basis of the data which I have obtained can say that 



IB 



I8 



bO 

 O 

 1^ 



Hb 



S.4 



40 



80 



Time 



120 



160 



Fig. 7. The temperature coefficient. Abscissae represent time in mm. along the 

 film; ordinates, logarithm of intensity. 



k for A (24°C.) = 0.775 

 k for B (16.5°C.) = 0.250 

 Ratio = 3.100 



