676 



FLOCCULATION OF BACTERIA BY PROTEINS 



tions between pH 4.4 and 6.4, with a maximum turbidity at pH 

 5.3 and 5.6, 



TABLE IV. 



Bacterium coli Suspension with Protalhumose. 

 1.0 cc. buffer mixture + 0.5 cc. protalhumose solution + 0.5 cc. coli suspension. 



Temperature = 40°C. All agglutinations were complete in 1 hour. 



TABLE V. 



Bacterium coli Suspension with Heteroalbumose. 

 1.0 cc. buffer mixture + 0. 5 cc. heteroalbumose solution + 0. 5 cc. coli suspension. 



Temperature = 40°C, X = agglutination within 30 minutes. + = aggluti- 

 nation within 3 hours. 



Flocculation with Edestin. 



Crystalline edestin was prepared from hemp seed by the method of 

 Osborne (1901). This substance was found to be practically in- 

 soluble in water or in the buffer mixtures used at reactions between 

 pH 5.6 to 9.6. Rona and Michaelis (1910) report pH 6.9 as the 



