716 



PHYSICAL CHEMISTRY OF THE PROTEINS. 



Although SO insoluble when uncombined it should be noted that 

 1 gm, of this preparation was completely dissolved by 1 X 10"^ mols, 

 of sodium hydroxide, or by slightly less than that amount of hydro- 

 chloric acid. The compounds of serum globulin with acids and with 

 bases are very soluble indeed. The smallest trace of a soluble com- 

 pound would have appreciably increased solubility. The measure- 



TABLE IV. 



Solubility of Serum Globulin Va. 



T. = 25.0° ± 0.1°C. 



ments reported must therefore have been upon the pure ampholyte, 

 upon the undissociated m.olecule and its dissociated ions. 



Certain proteins retain the power to dissolve in acid and alkaline 

 solutions even after becoming denatured. Denatured globulin will 

 not, however, dissolve in the solution of a neutral salt. No trace 

 of denatured material was present in this preparation. It readily 

 dissolved to a water-clear liquid in 0.1 n solution of sodium chloride. 



