JOURNAL 



SOTAfV ;■<.•■. A L 



Elisha Mitchell Scientific Society 



Volume XXXVI FEBRUARY Nos. 3 and 4 



PROCEEDINGS OF THE ELISHA MITCHELL SCIENTIFIC 

 SOCIETY, MAY, 1920, TO DECEMBER, 1920 



241sT Meeting— May 4, 1920 



W. deB. MacNider — On the Relation of the Amount of Stainable Fat 

 in the Renal Epithelium to the Susceptibility of the Kidney to the 

 Toxic Effect of the General Anesthetics. 

 Frozen sections were made from fresh kidney tissue and stained 

 for fat with Scharlach R by Herxheimer's method. Such tissue 

 when obtained from puppies and young dogs shows fat as minute 

 dust-Hke particles in the epithehum of the ascending Umb of Henle's 

 loop. Kidney tissue obtained from old and very senile animals 

 shows a marked increase in the amount of fat in the ascending limb 

 of Henle's loop and fine dust-like particles of stainable fat in the 

 convoluted tubule epithelium. Kidney tissue obtained from natur- 

 ally nephropathic animals shows a large amount of stainable fat not 

 only in the ascending limb of Henle's loop but also in the convoluted 

 tubule epithelium. When such animals of different age periods and 

 naturally nephropathic animals are anesthetized by ether, the relative 

 toxicity of the anesthetic for the kidney is shown in the following 

 manner : 



1. The puppies and young dogs continue to form urine during 

 the course of the anesthesia and remain responsive to diuretic solu- 

 tions. 



2. A certain number of the adult and senile normal animals 

 become anuric and fail to respond to diuretic solutions. Other 

 animals in this group remain diuretic for the earlier part of the experi- 

 ment and later become anuric. 



[103] 



