SOJIF. Or.SERVATTOXS ON TUYrAXOSOMA I5KUCET ? (PECAITni ?) 59 



110 rtagcllii. Their motility buciiiiie less active each succeeding day and, after the third i"-xpi-iiTiic_-ius 

 day, no typical trypaiiosome forras could be seen, all having assumed small spherical ^ „ ^^.• 

 or pear-shaped forms with long flagella attached. Some clumps of these forms were 

 seen on the sixth day slowly performing rotatory motions but no flagella were visible, 

 they having gradually shortened and finally disappeared. 



Subcultures were usually inoculated on the fifth day when the round forms were 

 present, but examination up to three weeks showed no recognisable trypanosomes. 



The culture work with this trypanosome is very incomplete as variations in the 

 proportions of blood and agar, advocated for other species, have not yet been tried, so 

 that nothing definite can be said, meantime, regarding the success or failure in cultivating 

 this strain. But the attempts made, their failure, and the forms met with up to a 

 limited period, correspond very closely to the cultural behaviour of T. ovand described 

 by Laveran and Mesnil. 



Note on Developmental Forms of T. brucei (pec.\udi) in the 

 Inteen.vl Organs, Axillary Glands and Bone-marrow of the Gerbil 



(Gerbillus rygargus) 



The transmission and maintenance of the trypanosome strains being under my care, 

 ample opportunities were afforded of noting the progress of the disease induced by them 

 in experimental animals and the structural changes in the trypanosomes themselves at 

 varying periods. It was, however, mainly the cultural work associated with this 

 trypanosome that led to further investigation of these developmental forms and thereby 

 established the observations forming the subject of this note. 



On beginning the culture work, inabilitj' to recognise if certain forms met with in 

 cultures were either developmental or degenerative phases, suggested a study of the 

 changes which the parasite undergoes, both in the body of the gerbil after death and in 

 citrated blood, and the comparison of these changes with the forms found in Novy and 

 MacNeal and Nicolle's media. With this in view, a gerbil was inoculated with infected 

 blood and chloroformed on the fifth day after injection. Cultures from the heart's blood 

 and smears from the various organs were made, all being examined at regular intervals 

 for comparison. Light was thereby thrown on the point in question, but, in addition to Xrypanosome- 

 this, attention was drawn to what undoubtedly looked like trypanosome forms in the 1''^'^ inclusions 



in the red cells 



red blood corpuscles in the spleen. Some of these were identical with those figured by of spleen 

 Chagas in his paper on Schizotrj/paniiiii cruzi, which appeared at the time this work was 

 being conducted. 



In order to coiifirm the observations made, a series of inoculations was carried out 

 in gerbils. 



In the first of the series, five gerbils were employed, these receiving subcutaneous 

 inoculation in the flanks. In each of the two later series, however, it was found 

 necessary to use eight gerbils at least, the injection being given intraperitoneally as 

 recommended by Chagas. The amount of infected blood in citrate inoculated into each 

 animal was 10 minims, all the eight forming one series receiving the injection at the 

 same time. A gerbil of the series was then chloroformed on each succeeding day, 

 beginning on the first day after injection. Films from the peripheral and heart's blood 

 were taken, and smears from the lung, liver, spleen, bone-marrow and axillary glands 

 were made, these being fixed and stained by various methods. 



