fiO N'OTF. ON m.;vi:i.nrMi'.NT.\r, 1'(1ums of TUvrANOsoMA uuicki (i-KfAfPi) 



Ihc iniia- As liotli the iiitni-corpuscular forms ami those wliiili possibly represent encysted 



corpiiscuhir sttttjos aiineareil in the si)leen on the fifth <hi\ in tlio first noi'bil, a careful examination 



forms apix-ar h 1 1 l ■ , , 



frum sixth to <'f tile films in the first series up to that tnne was mane, but none of the forms seen in 



ciRhth ilay (|,y oi-jj/injil slide were recoonised. In the other series, however, examination of spleen 



smears from f^erbils chloroformed on the sixth and seventh day respectively revealed 



both of the forms mentioned. These were also usually nbtaiiied in suljscipicnt ind(')icndpnt 



ruses (in the seventh and cif^hth day. 



^^01!IM^OI,Of; V AND ])KVF.I,OI'Mr.XT IN T H I'. Sl'LKKS 



(a) liitra-riirpHfciiliir. No definite merozoite forms as described by Chayas were 

 ever seen enteriufj; the red cells, and the small inclusion shown in Plate II., fig. 4, was 



KiiiR forms the only appearance observed whicli suggested a merozoite. Speaking generally, the 

 smallest intra-corpuscular forms met with took the shape of very small rings with two 

 chromatin masses, and were about one-third the size of the red blood cell. Nearly all 

 stages from this to a fairly mature trypanosome, contained within the limiting envelope 

 of the red cell, could be traced, the intermediate stages in many cases being similar to 

 those of Schi:otr\jpanum cnizi (Plate II., figs. 4-12). This fairly mature trypanosome, 

 however, possessed neither undulating membrane nor flagellum. Some of the more mature 

 forms were coiled uj) or S-shaped. every part of them being wholly witiiiii the corpuscle. 

 These varieties were not so numerous as the complete ring forms. The blepharoplast 

 was not recognisable in all cases, but, when present, w-as usually situated at the thinnest 

 part of the ring. 



(b) Extra-corpmcidar . Ring forms were also met with free in the plasma, and these 

 impressed one as being possibly encysted and likewise showed a development similar to 

 that seen in the red cell. Numerous small solid, encysted (?) forms appeared in the spleen 

 on the fourth day in one gerbil (Plate II., figs. 13 17), and these, as a rule, were also 

 present in other animals in the bone-marrow and axillary glands on and after the sixth 



-Spherical day. The very small types appeared as spherical masses of densely-stained blue 



protoplasm, the nucleus in some being indicated by a small undefined violet mass, while 

 the blepharoplast was detached. 



Round the spherical body here described there existed, as a ride, at least when 

 development was somewhat advanced, a clear area which gave the impression that the 

 protoplasmic mass was lying in some form of vacuoloid space which was possibly 

 surrounded by a limiting membrane. It was in connection with tliis mend)rane (?) that 

 the blepharoplast was found, hence the use of the term "detached" (Plate II., fig. lo). It 

 was found that, as the protoplasmic mass became larger, a clear area developed within 

 it. This increased in size, the nucleus of the mass became more distinct, and finally 

 the blepharoplast appeared gradually to approach the mass until it became part of it. 

 Such a movement on the part of the blepharoplast is well-nigh inconceivable unless there 

 was originally some connection between it and the main mass of protoplasm. Such, 

 however, I have never been able to demonstrate. 



Eventually the spherical mass<^s became ring-shaped, resembling in every respect 

 those met with in the red cells. 



These stages were always observed within the vacuoloid space wliich eidarged to 

 accomuiodate the requirements of the developing trypanosonie. As in the red cells, forms 

 almost mature were eventually found. These, wliich possessed free extremities, often 

 assumed S shapes within a faintly defined capsule, the nucleus and blepharoplast 

 being situated as in an ordinary trypanosome. Later observations showed that these 



bodies 



