NOTE ON DEVELOPMENTAL FORMS OF TRYPANOSOMA BRUCEI (PECAUDi) 



61 



forms were invariably found free in the plasma of the spleen, bone-marrow and axillary 

 gland several days previous to the appearance of the intra-corpusculai' forms. 



Forms found 



Lung Smears 



The probability of schizogony occurring in the lung as in the case of Schizotrypanmn 

 cnizi was borne in mind, but no definite schizonts were seen in any of the smears. Yet the 

 forms figured by Chagas,' and described by him as parasites in the lung of vertebrates 

 preparing for schizogony were exceptionally numerous in the lung of a gerbil on the 

 sixth day. In fact they were the only forms present in smears from the lung at that time, 

 but no suggestion of merozoite formation was ever observed. Some forms seen resembled 

 the so-called latent bodies of T. gambiense described by Moore and Breinl,- and more recently 

 by Fantham,^ as occurring both in T. rjamhiense and T. rJwdesiense infections. Their method 

 of formation, however, seems to be somewhat different to that described by these authors. 

 It is, jjerhaps, sufficiently indicated by the cycle of events shown in Fig. 17. In 

 addition there was markedly evident the presence of individual spherical forms dividing Presence of 

 into two, and occasionally three, separate bodies, each of which eventually assumes a "'^"^ ^^^' 



' ■ '^ " young 



trypanosome shape. As a result, large numbers of what were undoubtedly very young trypanosomes 

 trypanosomes, each with a well-defined nucleus but without undulating membranes and '" '""g-fiims 

 flagella, were present in the lung smears. 



r 



y^. 



f 



O. BUCHiKiU 



X apprax. 2000 diatii. 



Apparent fonnatioo of non-fla^ellate body from trypaaosome in lung 

 apparent metamorphoKis of non- flagellate body into trypanoaoine in King 



No definite intra-corpuscular or free encysted forms were ever seen in the lung or 

 peripheral blood. The former appeared exclusively in the spleen while the latter were 

 found without fail in the spleen, bone-marrow and axillary glands. 



The axillary glands showed an unending variety of young trypanosomes corresponding 

 in the main to those described above in the lung, while the encysted stages were also very 

 numerous. 



A marked feature, perhaps worthy of note, was the constant presence of free chromatin Free 

 granules in nearly all the smears from the second dav onwards. These were apparently i^hromatin 



T ■ 1 /• 1 " 1 ■ p T ■ ■ " ' "granules" 



derived from the nuclei of disintegrating trypanosomes and possibly represented a first 

 stage in development. 



* Chagas, C. (August, 1908), " Uber eine neuc Trypanosomiasis des Menschen." Memorias de Instituto 

 Oswaldo Cruz, Vol. 1, No. 2. 



= Moore, J. E. S. and Breinl. Anton (.July, 1907), " The Cytology of the Trypanosomes." Annals, of Tropienl 

 Medicine and Parasitohir/;/, Vol. 1. 



' Fantham, H. B, (1910), "The Life-history of Trt/pnnosomn yimihienise and Ti-iipnnosuma rluidesiense as seen in 

 rats and guinea-pigs." Proceeding!: of lioyal Society, B. Vol. 83. 



