

PLATE III 

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1. Semi-diagrammatic and composite drawing from smears of portions of one Malpighian tube of a tick (Ar^as />frsittts 9^ 

 incubated for 23 days at 37° C. after feeding on a chick with acute spirocha;tosis. Small clumps of Leishman's spirochsete granules 

 and transition of granules into spirochaetal forms. The two large nucleated granular cells in the middle are normal tissue cells 

 and are introduced by way of contrast. 



Lcishiuan Stain 



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X 1000 i/iam. 



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2. Heavy gr;inule infection at different stages 



3. Inclusions showing "spore" forms discharging "spores" 



4. Clump of spirocha;tes with central node (peripheral blood) 



5. Spirochaetes from coclemic fluid of infected Ar^as ptrsicus 

 2-7. Lcishman Stain 



8. Iron Hiematoxytin and SaJ/ranin Stain 



Spii-ochfCta granniosa penetrans, ii.sp. 

 2 — 8. Cycle of Schizogony 



Gaps in red cells after discharge of "spores" and 



inclusion with attached rods 

 Inclusions and free spirocha;tes in apposition 

 Multiple infection of red cell-especial staining 



X 1000 iiiant. 



