234 



Forn CASES of pyrexia 



(ipiKiuc centre surroiuiflod l)y a translucent rim. A similar appearance was produced 

 on lOndo's fuclisin-agar. Acid was not produced in either of those media. On Faweus's 

 medium tlic {growth ajipearc'd as minute translucent dew-drop like colonies resembling 

 somewhat in appearance those of H. tiipJidsnx. Aj^ar slopes inoculated with tliis orj^anism 

 showed a •jreyish-white lihn with irrej,'iilar marj^ins. At the end of 24 hours the growth 

 in this medium was not a luxuriant one. In lirotli tlic oii,'aiiism jjroduced a cloudiness 

 without the formation of a pellicle. 



Hanging-drop preparations taken from a young culture showed tlie presence of a very 

 small, actively motile organism of the nature of a cocco-bacillus. Stained preparations 

 showed a cocco-bacillus measuring on an average 1'6// in length. Some of the larger 

 .Mnrphoinsy fomis uieasurod TH/i. In agar cultures the oi'ganism assumed more the type of a small 

 bacillus, tiie coccoid forms having disappeared. It was easily stained l)y most of the basic 

 aniline dyes, was Gram-negative, non-acid fast and non-spore forming. Its optinmm 

 temperature was 37 C, while its minimum and maximum temperatures were 32 C. and 

 52 C. respectively. It was a facultative anaerobe. On blood serum it produced a yellow 

 creamy growtii, l)ut no digestion occurred, wliilc on ])otato, minute translucent colonies 

 appeared at the end of 4S hours. At the end of a week these were somewhat huff-coloured. 



It was non-pathogenic to guinea-pigs and rabbits. In the latter a distinct rise of 

 temperature occurred and lasted for 4 days. The specific sera of tijphoid, paratyphoid A. 

 and parati/phoid B. failed to agglutinate it. 



The following reactions occurred in the various sugars, etc. : 



+ = Acid and gas. 

 A. = .\cid onlv. 



— — No acid and no gas. 

 ALK. ^ .Mkalinitv. 



Cultural 

 characters 



In litmus milk there was slight acidity after three days. .\t the I'nd (jf leu days, no 

 clotting had occurred, Init tlie litmus was totally discharged, the milk remaining white 

 in colour. At the end of a month the reaction of the medium was alkaline. 



Oelatine as a culture mcclium could not be employed owing tn the existing climatic 

 conditions. 



.\lthough the cultural reactions of this organism did not correspond with those of 

 the li. tiipliimiis and the paraf uphold (/nmp, the fact that it might inive been a modified 

 variety of them justified further differentiation tests being carried out. Three rabbits were 

 immunised by injections of killed cultures of li. fi//ih(iK}is, li. pamfi/phofnii li. and the recently 

 isolated organism. 



A series of agglutination tests was then carried out between tiie sera of the animals 

 innnunised against li. Ii/plm^ntx and li. p<iriiti//)h<isiiii li. and the new organism in question. 

 The agglutination tests were made by the macroscopic metliod. Instead of broth cultures, 

 emulsions made in normal saline solution, from agar cultures grown at ."{7 ('. for '24 liours. 



