LYCtEXA II., III. 13 



not till the latter part of the same stage to emit the secretion. The ants, when 

 confined with larva? in the first stage, treat them with indifference. I introduced 

 ants to larvaj in separate gla.ss tubes, some larva? being at the middle and some 

 near the end of the second stage ; that is, before the second moult. One of the 

 larva? was caressed several times, but no tube appeared. One larva ol))ected to 

 the ant, thrashed its anterior segments about, and the ant left it. Introduced ant 

 at oue day after third moult ; ant, greatly excited at finding itself imprisoned, ran 

 about the tube, often coming close to the larva, even touching it, and then ran 

 across it, apparently taking no heed of it. But after the ant had quieted, it came 

 to larva, soliciting as usual. Then the tubes were seen, and a drop of fluid came 

 from 11, which the ant drank eagerly. It returned to 11 several times, but ob- 

 taiued no more. On same day, introduced an ant to two larva^ in last part of 

 third stage ; that is, just before the third moult. There was a slight movement of 

 the tubes in one larva, a mere point protruding, but no more, and no fluid. The 

 other larva did not respond at all, and the ant left both. 



But on another day, experimenting with a larva also in third stage, the tubes 

 were .seen to play actively. I could not make out a drop on 11, but the ant held 

 its mouth at the orifice for some seconds. It is after the fourth moult, in the last 

 larval stage, that the fluid flows freely at the solicitations of the ants. This occur.s, 

 however, only with the summer larvte, on Eattle-weed, the flower of which is of 

 exceeding sweetness. I do not remember to have seen an ant on a Dogwood 

 flower but in a single instance, and when ants are placed with Dogwood larvfe 

 they soon become indifferent to them. Just so, in the fall, when the larvJB are 

 feeding on Actinomeris, which has a dry flower, bitter to the taste. I have 

 seen au ant approach a mature larva on this plant, and after a moment's investi- 

 gation turn away. My observatious in 1S7S led me to think that the tubes 

 served as signals to the ants to approach segment 11, and subsequent observa- 

 tions afford no better explanation. I have observed in larva? of last stage, Avhere 

 no ants were present, that the tubes were occasionally but irregularh' protruded, 

 one appearing without the other, or both at same time, a very little or almost 

 fully out, but without the tentacles .spreading ; and at such times I never could see 

 any movement at 11. The presence of the ants seems necessary to that; that 

 is, the larva emitted the fluid when aware that its friend was near. Anv teasingr 

 on my part failed to provoke a discovery of the tubes, as seems natural, for they 

 certainly are not for defense. At least four species of ants accompany these lar- 

 va?, the smallest of which is scarcely' one eighth inch long. The gentle demon- 

 strations of these little creatures were most interestini;:, fis if askino: favors but 

 claiming no right, and grateful for wdiat they got. 



In 1879, Dr. J. Gibbons Hunt kindly made observations on these larval organs, 



