Protista. 253 



in the hinder part of the mid-gut of tlie fly. These crithidia are described by 

 Miss Robertson. The kinetonucleus appears to be double, even when still 

 connected with a sister- kinetonucleus by a centrodesmus — i. e. immediately 

 after division. A rather posteriorly-situated granule, similar to that met with in 

 development stages of T. raiae, was also noticed, and in some cases appeared to 

 be attached by a fine line to the nucleus or to the blepharoplast, or even (by 

 two lines) to both. Division is usually binary, but may be irregularly multiple. 



Miss Robertson states that G.palpalis is certainly not the normal host of 

 T. gallmarum, and is probably not even a facultative host. A small Simulid fly 

 is suggested as being possibly the true transmitter. 



Duke has tested Muscovite ducks and found them incapable of acting as a 

 reservoir for T. gamhiense. Ducks which were proved cleau by feeding flies on 

 them and afterwards examining the flies, were fed on by infected flies. No flagel- 

 lates were found in clean flies subsequently fed on the ducks, nor did micro- 

 scopic examination of blood, nor injection of it into a susceptible animal, yield 

 any positive result shewing that the ducks had become infected. 



Lewin (Cambridge). 



836) Dönitz, W. (f), Die Bekämpfung der Schlafkrankheit. In: 43. Ber. Sencken- 

 berg. Naturf. Ges., S. '2'J5— 323. 1 Karte, 15 Abb,, 1912. 



Be.sonders betont wird die Schwierigkeit der Bekämpfung, die aus der Natur der 

 Neger, ihrer Lebensweise und ihren Anschauungen erwächst. Der ganze Feldzug gegen 

 die Schlafkrankheit kann sich nur aus verschiedenen „kleinen Mitteln" zusammensetzen. 

 Durchgreifende Maßnahmen sind zurzeit ausgeschlossen. Die Kai-te zeigt die Verbreitung 

 der Schlafkrankheit und von Glossina palpalis. Loeser (Dillingen a. d. Saar). 



837) Duke, H. L., Further Observations on the Recovery of Trypano- 

 soma gambiense from Tragelaphus spehei on the Islands of Lake Vic- 

 toria Nyanza. In: Proc. Roy. Soc. London, VoL B. 85, S, 483—486, 1912. 



Additional evidence is brought forward that the' trypanosome recovered 

 from antelope on Damba Island (Duke, Proc. Roy. Soc. B. 85, p. 156) was T. 

 gambiense and not T. brucei. By inoculation into a goat, one infection by T. vivax 

 was discovered in a situtunga shot on a visit to these Islands. The conditions on 

 these Islands are less favourable than on Damba to the perpetuation of sleeping 

 sickness by antelope. Lewin (Cambridge). 



838) Bayou, H. (Lister Institute), The Cultivation of Trypanosoma rho- 

 diense Stephens andFantham. In:Proc.Roy.Soc.London,VohB.85, S.482 — 483, 

 1912. 



Trypanosoma rhodiense was successfully cultivated on 



(1) Clegg's Amoeba agar to which is added twice its amount of rabbits' 

 blood which bas been frozen and thawed rapidly so as to cause the haemoglobin 

 to diffuse into the serum. 



(2) A medium of the following formula: 



Agar 15 grm 



Glucose 10 „ 



Water 1,000 „ 

 and twice the volume of rabbits' blood added. The majority of the cultures were 

 unsuccessful. Lewin (Cambridge). 



839) Bruce and others, The Morphology of the Trypanosome causing 

 Disease in Man in Nyasaland. In: Proc. Roy. Soc. London, VoL B. 85, 

 S. 423—433, 1912. 



