2 



Transartionfi. 



that the results so obtained give no indication of the commercial value of 

 the extract. 



The following additional methods were used in this investigation : — 

 Acidity. — Titrate a solution of 0-5 gram in 800 c.c. of water with deci- 

 normal potassium-hydrate, using phenolphthalein as indicator ; the result 

 is then calculated and expressed in terms of acidity as lactic acid. 



Insoluble and Coaijuable Proteids. — For these determinations the provi- 

 sional methods of the Association of Official Agricultural Chemists for meat- 

 fibre and coaguable proteids were used.* 



Proteoses were determined by the zinc-sulphate method. f 

 Peptone-like bodies and jjohjpeptides were precipitated together with the 

 proteoses by the tannin-salt reagent, the amount of peptone-like bodies 

 and polypeptides being found by difference. { 



Total Meat Bases. — From the nitrogen found in the tannin-salt filtrate 

 deduct the nitrogen found as ammonia, the difference being the nitrogen 

 as meat bases. 



Ammonia was determined by the magnesium-oxide method. § 

 Purin bases were determined by Schittenhelm's method.!; 

 Kreatin and kreatinin were determined by FoUin's colorimetric method.^ 

 Phosphoric acid and potash were estimated in the residue from the ash- 

 determination.** 



* U.S. Dept. of Agric.. Bureau of Chem., Bull. 107, p. 115. 

 t Ibid. 



X Journ. Amer. Chem. Soc., 190(), vol. 28, }). 148."). 

 i? U.S. Dept. of Asric, Bureau of Chem., Bull. 107, 

 II U.S. Dept. of Agrie., Bureau of Chem., Bull. 110. p. 120. 

 il Zeit. Physiol. Chem., 1004, vol. 41, p. 223. 

 ** U.S. Dept. of Agric., Bureau of Chem., Bull. 107, })p. 3, 4, 



0. 



md 11. 



