496 JOURNAL OF ECONOMIC ENTOMOLOGY [Vol. 9 



dried out, and fresh alfalfa was supplied as necessary. All cages were 

 placed in a conservatory having two walls glassed in and connected 

 with a room of the laboratory, thus insuring more light and circulation 

 of air than is ordinarily found indoors. All cages were disinfected 

 before use by being washed in 20 per cent carbolic acid and rinsed in 

 tap water. Infection was, with the exception of the first experiment, 

 by introduction into the cages of insects showing well developed fruit- 

 ing fungus. Alfalfa not eaten by weevils was allowed to accumulate as 

 debris in the bottom of the cages. 



Experiment I 



March 23 : Five weevils were infected by contact with a fungus-covered weevil in a 

 moist chamber for 2 hours. They were then placed in a battery jar cage partiallj- 

 filled with moist sand and alfalfa was introduced as food. 



Aprils: One weevil dead. 



April 7 : Second weevil dead. 



April 9: Third weevil dead. 



April 10 : Fourth weevil dead. 



April 25: Fifth weevil dead. 



All developed a pure growth of SporotrirMmi globuUferum within two to three 

 days of death. 



■Experiment II 



March 2.5: Thirteen weevils were placed in a small vial with a fungus-covered 

 weevil and left in a moist chamber for 4 hours. The weevils were then placed in a 

 tight tin tobacco box partially filled with moist sand. Alfalfa was placed in the cage 

 as food. Later a weevil showing a good growth of fungus was placed in this cage 

 under the alfalfa. 



March 30: Three weevils dead, 2 from fungous disease; 1 from unknown cause. 



April 3 : One more weevil dead. A living weevil which seemed to be ailing was dis- 

 sected. The blood of the abdomen contained several fungus hyphse of various 

 lengths. The blood of the thoracic region showed more numerous fungus hyphae 

 and these were generally longer and further advanced than those in the abdomen. 

 Incipient branching of the hyphae was observed in several cases. Fungus bodies 

 practically identical with the "cyhnder-gonidia" observed in Van Tieghem cells 

 were observed in the blood. 



April 6 : Contents of cage were examined. Found 12 dead weevils. 



All developed typical fungus, except the weevil noted March 30 as dead from un- 

 known cause. 



Experiment III 



April 5 : Twelve weevils were placed in a tobacco-box cage with a fungus-covered 

 specimen. Moist sand and alfalfa were used as in Experiment II. 



April 6: One weevil dead of fungus. This weevil was probabl}^ infected when col- 

 lected. 



April 8 and 1 1 : One weevil was dissected on each date without finding signs of 

 fungus. 



April 15: All weevils dead. 



April 21 : All developed typical fungus except the two dissected. 



