October, '16] rockwood: sporotrichum enemy of alfalfa weevil 497 



Experiment IV 



April 7 : Twenty weevils were placed in a tobacco-box cage as before with moist 

 sand. 



April 8: One weevil dead from unknown cause. One living weevil was dissected 

 but no signs of fungus were seen. 



April 15 : Two weevils dead. 



April 16: An enfeebled weevil was dissected. The abdomen and thorax were 

 well supplied with fungus hypha> varying from " cylinder-gonidia" to long, consid- 

 erably branched hypha>. 



April IS: A dead weevil showing no exterior growth of fungus was dissected. The 

 body was found to be packed with a reddish-brown mass of fungus hyphse. 



April 20 : All weevils dead but one. 



April 21: Last weevil dead. 



All not dissected developed typical fungus. 



Experiment V 



April 16: Fifty weevils were placed in glass battery-jar cage, the bottom of which 

 ■was covered with garden loam. The cage was infected by placing in it 4 fungus- 

 covered specimens. 



April 21 : Twenty-five more weevils placed in this cage. 



April 28 : Weevils dying in numbers. 



May 7: All weevils dead. 



Experiment VI 



May 8: Thirty weevils were placed in a battery-jar cage with garden loam as 

 before. The cage was infected by the introduction of several fungus-covered spec- 

 imens. 



May 18: All but 3 dead. 



May 20 : All dead and all developed typical fungus. 



Experiment VII 



Aug. 7: Twenty-two weevils reared from pupse about July 15 were placed in a 

 battery-jar cage with moist sand. Cage was infected by introducing several fungus- 

 covered specimens. 



Aug. 20: Several weevils dead. 



Aug. 31: A few still alive. 



Sept. 18 : Four weevils still alive. 



Oct. 13 : Last weevil dead. 



All developed typical fungus. 



Experiment VIII 



Sept. 30: Fifty weevils collected from a ditch bank, were placed in a battery-jar 

 cage with moist garden loam; infection brought about as in previous experiment. 



Oct. 14: Three dead. 



Oct 20 : Many dead and showing fungus. 



Nov. 4: Forty-five weevils dead of fungus, 4 weevils alive, 1 lost. 



Nov. 18 : One weevil still alive. 



Nov. 20: Dissected the last living weevil. Found a few scattering Sporotrichum 

 "cylinder-gonidia" in the blood. Most of them were of irregular or indistinct out- 

 line as if undergoing cytolysis. 



This cage contained 47 weevils dead and showed typical extei'ior growth of S. 

 globuliferum, 1 weevil dead with body filled with the hypha; of the typical fungus, 1 

 weevil with growth of Aspergillus sp., probably dead from some other cause. 



