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division of a single element is marked. But often there are irregular groups 

 of three or more elements, or there may be a chain of tetrads which remain 

 attached one to the other. 



"In agar stick cultures, a rather thick and viscid yellow mass is 

 formed on the surface, about the point of puncture; and in the course 

 of a week or ten days, at a temperature of 20° to 25° C, this extends over 

 the entire surface. The colour varies from cream yellow to lemon yellow. 

 The growth upon potato is similar to that upon agar. In stick cultures in 

 flesh-peptone-gelatine, the gelatine is liquefied rather slowly near the 

 surface, forming a deep cup-shaped cavity, as shown in Fig. 7, PI. III. 

 Colonies in gelatine roll tubes are at first pale yellow, and later, lemon 

 yellow in colour; they are opaque and spherical, and do not usually cause 

 the liquefaction of the gelatine for several days. 



"The micro-organism has no proper movements, is aerobic, no growth 

 occurring in an atmosphere of hydrogen. It is not pathogenic for rabbits 

 or guinea-pigs." 



Before taking issue at this last assertion of Dr. Sternberg's, I beg to 

 be allowed to point out some details in "technique," which may be useful 

 to those who desire to repeat my experiments. Young colonies in agar 

 seldom present characteristic tetrads; these are easily obtained, however, 

 by planting the same colony into broth or some other liquid medium. 

 In order to identify our microbe, it is not, however, necessary to find 

 it in tetrads ; all that is required is to determine the characteristic 

 segmentation of the protoplasm of each individual element. It happens that 

 the same elements which with an ordinary magnifying power appear 

 simple cocci, when vitwed with the strong oil immersion objetives y 1 ^- — -Ar 

 of Zeiss or Leitz) are found to consist of two elongated semilunar masses, 

 which are well coloured by fuehsin or methylene blue. At the first stages 

 of this segmentation we have the appearance of a semitransparent line or 

 cleft extending along one of the diameters of the coccus which a confrere 

 has aptly compared to a coffee-bean with the furrow dividing its plane 

 surface. As the germination proceeds, the cleft, or rather the pale-coloured 

 intermediate substance, widens, and the two segments become rounded or 

 lengthened, one or both undergoing, in the meantime, a new segmentation 

 in a direction perpendicular to the primary division. A group is thus 

 formed consisting of four cocci of round, oval, or unsymmetrical outline, 

 constituting a square or tetragon. In some preparations the group is 

 evidently enclosed in a distinct capsule, in the interior of which the growth 

 and subdivision of the elements may continue for a while; but it more 

 frequently happens that both the tetrads and the "coffee beans", arranged 

 in chains or otherwise, are found naked. It is also worthy of notice that, the 

 cleaving of the protoplasm is observed in the small as well as in the large 

 cocci which are seen in the same preparation. With regard to the grouping 

 of the elements, there is another rather characteristic arrangement which 



