315 



of albumen. Two tubes planted with heart blood gave pure cultures of the 

 tetracoccus. 



On July 1, I planted two tubes of glycerine broth with blood of a 

 patient (B. V., female) in the fourth day of yellow fever (typical non- 

 albuminuric form). She was a wet nurse. On the same day I introduced 

 into an agar tube some of her milk pressed directly from the nipple, which 

 I had previously washed with soap and water and with absolute alcohol. 

 The tubes planted with blood germinated, giving only straw-coloured 

 ietracoccus, and the agar tube presented, all along the track followed by 

 the milk, an abundant growth of white tetracoccus colonies, and no others. 

 Rabbit P, inoculated with 7^ c. c. of this white tetracoccus from human 

 milk, in a five days' culture produced a reaction of 41° C. on the same 

 day, followed by two days without fever, and invasion of the regular 

 attack on the fourth day after the inoculation. The fever showed a 

 remission on the third day, and defervescence on the eighth ; the animal 

 continued, however, losing flesh, and died on the fifteenth day after 

 the inoculation. No autopsy nor cultures were made. 



On July 2, I planted two tubes of glycerine broth with finger blood of 

 a yellow fever patient (a simple albuminuric case) in the fourth day of the 

 disease, occupying bed N.° dy 2 of Dr. Scull's ward in the Mercedes 

 Hospital. Both tubes germinated, giving the straw-coloured tetracoccus. 

 On the 6th and 10th of the same month rabbits N and O were inoculated — ■ 

 the first with 9 c. c. of a forty-two hours' culture, and the second with 10 

 c. c. of a fifty -six hours' culture of the tetracoccus from this source. N 

 showed a febrile reaction of 40° 7 C, followed by a two-paroxysm fever, 

 with a remission below 39° C. on the sixth day but without notable loss in 

 weight. Defervescence took pbace on the fourteenth day ; the animal 

 recovering definitely. At the end of twenty-seven clays the original weight 

 of 860 grammes had increased to 980, being the only one of my inocclated 

 rabbits which did not succumb to the inoculation. The other rabbit, O, 

 which had received a larger amount of a more developed culture, showed 

 a febrile reaction of 40° 6 at the end of seven hours, followed by a fever 

 of the same type as N's, with a remission from the fifth to the sixth day; 

 but the loss of flesh became gradually more marked, evolving into a chronic 

 process which caused death at the end of twenty-three days, the loss of 

 weight being from 750 to 575 grammes. No autopsy nor cultures were 

 made. 



Finally, on the 9th of August, I planted two tubes with the finger blood 

 of a yellow fever patient (severe albuminuric type) in the fourth day of the 

 illness. Both tubes germinated, developing pure cultures of yellow tetra- 

 coccus, more intensely coloured and more virulent than the preceding ones. 

 Ten c. c. of a four days' culture of this tetracoccus inoculated into rabbits 

 Q and R produced on the first and second days a febrile reaction of 40° 2 

 and 40° C ; Q dying in the first paroxysm of this fever thirty-eight hours 



