The results of the survival experiment indicated: 



1. that the salmon viscera diet gave no evidence of being deficient 

 in either the anti-anemia factors or thiamine. A lower mortality 

 rate resulted frcan the salmon viscera diet than from any of the 

 other diets. 



2. that the acetone-extracted salmon viscera meal and low-tanperatu re- 

 dried salmon viscera mead, were deficient in thiamine and that the 

 acetone extraction procedure removed more of the thiamine than did 

 the procedure employed for the preparation of the low-temperature- 

 dried meals . 



3. that the addition of thiamine nydrochloride to the diet was success- 

 ful in alleviating the symptoms of the thiamine deficiency, 



4. that the anti-anemia factors known to be present in the raw salmon 

 viscera were not retained in adequate amounts in meals prepared by 

 the acetone- extract ion process. 



The gi'owth evaluation investigations indicated: 



1. that salmon viscera, including the eggs and testes, contained an 

 excellent growth potential y&iea fed either as the single diet com- 

 ponent or in conjunction with meat products. Observations pre- 

 viously made were substantiated that, for the 12-week experimental 

 period, ths salmon viscera diet permitted better growth and lower 

 mortality "ihan the 100 percent beef ]J.ver diet. 



2. that low-temperature-dried salmon viscera meal, acetone-extracted 

 salmon viscera meal, or flame-dried salmon offal meal when fed 



at the 10 percent level in the diet each made a highly significant 

 contribution to the grcwth rate. 



3. that tesnperatures up to 150° F., when used to dehydrate salmon 

 viscera, do not alter the protein in such manner as to make it un- 

 available to fish, 



4. that intense heat such as that used in the dehydration of flame- 

 dried salmon offal meal has no effect on the growth potential of 

 the meal. This conclusion, although indicated in the statistical 

 analysis of the data, is subject to further corroborative evidence 

 because of the introduction of an additional variable and the short 

 term of the exparimental period. 



5. that salmon waste, minus the viscera, produced very poor growth 

 rates and that the fish fed this diet were on the verge of an acute 

 anonia at the end of the 12-week experimental period. 



6. that frozen salmon cannery waste (visceral portions) is superior 

 to beef liver as a food for growth. Methods attempted to make the 

 salmon viscera non-perishable without refrigeration (i.e. dehy- 

 dration without heat by means of acetone extraction and air drying 

 at 150° F.) caused serious losses of nutritional factors and some 

 other preservation methods will have to be found if freezing and 

 cold storage is dispensed with. 



5V 



