Suamary 



Fish meals to t-8 used in hatchery feeding tests wert. prepared 

 from cannery waste materials. Those used were Columbia River Chinook 

 saijtton viscera, Alaska pink offal^ and partially dried Puget Sound 

 pink viscera. The meals were prepared by cooking to coagulate the 

 protein and then drying in air at 100° F. and 145°F., and by acetone 

 extraction at room tonperature. Some of the Alaska pink waste was 

 allowed to spoil and was then oooked and air dried at 100°F, Vitamin 

 B and proximate analyses were run on the raw materials and meals as 

 well as the stick water con cent I'ate and samples of the hog and baef 

 liver and hog spleen being used at the Leavenworth Hatchery. Micro- 

 biological assays were used to determine niacin and riboflavin and 

 the flnorcmetric method in the deteimination of thiamine. 



There was no great difference between the analyses of the meals 

 dried at 100°F. and 1/».5°F., but the vitamin content of the flame-dried 

 meal was lower. Many of the vitamins lost during the processing of 

 the meals were dissolved in the stickwater concentrate. The acetone 

 extraction of the fish waste resulted in a lower vitamin content of 

 the meal than that of the air-dried meals. Hog spleen had a lower 

 protein and vitamin content than either the hog liver or beef liver, 

 Salmon viscera had approximately the same amoxint of protein, but a 

 lower vitamin content than the beef liver. 



Literature cited 



1. THE ASSOCIATION OF VITAMIN CHEMISTS. 



1947. Methods of vitamin assay. Interscience Publishers, Inc., 

 New York City. 



2. ROBERTS, ELIZABETH CUNNINGHAM, and SNELL, ESMOND E. 



1946. An improveu medium for micirobiologioal assays with 

 lactebaclllus casei. Jour, of Biol. CLem. 163 . 

 No. 2, 499-509. 



EVALUATION OF SALMON HEAD OIL FOR ADDITION TO CANNED SADION 



By Charles E. Eutler 1/ 



Introduction 



For many years salmon oil, prepared from fresh salmon heads and 

 collar bones, has been added to canned saluon. This practice began 0© 



1/ Chemist, Fish eiy Technological Laboratory, Fish and Wildlife Service, 

 Seattle, Washington 



89 



