Prefecture using sardines and anchovies^, and experimenting in the natural 

 order, attempted to find first whether or not the sardine is completely- 

 lacking in the ability to regulate its body temperature , and then to what 

 extremes of high and low temperature the sardine can maintain life, 



a) Chemical evidence of the fact that the sardine does not have the faculty 

 of regulating its body temperature^!!! 



In the homiothermie animals, from the birds on up, the temperature 

 center is supposed Ln and around the tuber sinereum of the hypo~ 

 thalamus behind the hypophysis,, The fact that when this area is 

 subjected to cold and heat stimuli it brings about a raising or lowering 

 of the body temperature has been gradually revealed on the basis of the 

 cooperative studies of Isenschmid and KrehlJ-6) a Furthermore, according 

 to Meyer^'^ aninu dch have a body temperature regulating center 

 develop fever when injected with sympathetic nerve poisons and when 



parasympathe e poisons are injected, their body temperature goes 



down ■== an interesting facto Because of this tne author decided to leave 

 the anatomical study of the temperature center to some future time and to 

 determine by Meyer's method tnrough the reactions to injections of nerve 

 poisons whether after all the sardine lacks the capacity to regulate its 

 body temperature, or in other words, whether the sardine does not after 

 all nave a temperature center,, 



First of all subcutaneous injections were made in sardines using 

 very small amounts (0,2 - 1,0 cc) of a 5$ solution of the hydrochlorate 

 of ac=tetrahydro=^=napntylamin, a chemical which markedly excites the 

 temperature center in the diencephalon and other sympathetic nerve 

 ganglia and brings about centrally tne production of high fever, of very 

 small amounts (0^2 = o 5 cc) of a quadruple solution of the hydrochlorate 

 of adrenalin, which operates centrally and peripherally to cause fever, 

 and of very small quantities (average 0,5 cc) of pituitrin, which works 

 centrally to lower tne body temperature* After injection the body 

 temperature was determined by inserting a fish body temperature thermo~ 

 meter into the esophagus (this is a mercury thermometer with a fine, 

 more or less curved tube and a scale which can be read to an accuracy 

 of 1°C, manufactured by the physical and Chemical Instrument Manufacturing 

 Corp )° As a result, of repeating these experiments several score times 

 it became clear that the body temperature of sardines cnanges with the 

 temperature of the surrounding water and aside from that such drugs 

 cause absolutely no rise or fall in the body temperature, that is, no 

 pharmacological changes in the body temperature can be detected* However, 

 the author would like to note here his misgivings on the score that even 

 supposing that a slight fever had been produced oy the action of the 

 above-mentioned drugs, it would probably have been impossible to detect 

 it because it would have been immediately dissipated by the conduction 

 and radiation of the heat, Nevertheless, if there nad been any fever 

 produced by the drugs, such fever would ordinarily have continued over a 

 rather long period of time, and even though the degree of fever might be 

 slight, its accumulation should reach an amount which could be measured, 



VIII. The kind assi ;chi cian Migita and Attache Komoda was 



obtained in the preparation of enemicais and in other work. 



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