gave 4,000 grams of groundup sardines to some live bait (50,677 fish) 

 that had been kept for 47 days since their capture without feeding. In 

 16 - 18 hours after the feeding many dead fish appeared, all of which 

 were gorged with food^)-. 



In healthy fish swimming in the broad sea, a sardine 15 cm long 

 ordinarily has 1 gram or less of material in its digestive tractl5) 33) ^ 

 but for fish inside a small bait tank, with a restricted scope for move- 

 ment;, this appears to be a little bit too much* The author thinks that 

 the right amount is such that there is no food in the cardiac portion 

 or the blind sac of the stomach, a little in the pyloric part, and a 

 moderate quantity in the intestine, as shown in Figure 14, that is, so 

 that the food does not remain for long in the stomach. When food is 

 suddenly to be given to bait fish that have not been fed for a long time, 

 care should be taken to give them a very small amount at first and then 

 to gradually increase the quantity,, 



On Vomiting in Bait Fish 



If bait fish are placed in bait tanks immediately after capture 

 and transported, the water inside the bait tanks becomes turbid with 

 their excreta, at times to such a degree that the water snows a yellow 

 color. The fishermen explain that this phenomenon is the result of 

 vomiting by the fish, but the author could not help having considerable 

 doubt as the presence of a vomiting center in lower vertebrates such as 

 fish, and therefore it was decided to try the following experiment 

 Small amounts (0 o l - C e 3 cc of a 8 5$ solution) of apomorphin= 

 hydrochloride, the only drug that can be injected to stimulate the 

 vomiting center and cause forced vomiting when human beings have drunk 

 poison with the object of committing suicide, were injected into the body 

 cavities of bait fish shortly after feeding and the reaction was observed. 

 As the following table shows, it was found that, the fish regurgitate 

 conspicuously,, (The same reaction is found in mackerel-scad, 

 Chelidon ichthya kumu , mackerel, £?hasticht-hys el^UST 1 ^ anc * Sebastodes 



Thus it was found to be possible that a vomiting center exists in 

 sardines (fish in general) „ Since a vomiting center is easily excited 

 by spiritual agitation^S) ^ there is always a possibility that the bait 

 fish, which receive mental shocks during their capture, may regurgitate 

 immediately after being placed in the bait tanks. Not only is the fish 

 itself considerably weakened by vomiting, but the material regurgitated 

 immediately causes a change for the worse in the character cf the water, 

 and for this reason, even though the vomiting itself may not be related 

 to the death of the bait, every care is required in handling these fish. 



Death of Bait Fish Exposed to the Air 5 * 5 ) 



In placing bait in bait tanks at present dip=nets or buckets are 

 used, and several dozen separate transfer? are made. At this time quite 



4S 



