3 percent 5,2' -dichloro-4-nitro- 

 salicylanilide and 99 to 97 pe rcent 

 of 3-trifluormethyl-4-nitrophe- 

 nol. These two compounds form a 

 synergistic combination which 

 increases toxicity to larval lam- 

 preys without destroying selec- 

 tivity. 

 Inventors: John H. Howell and Everett L. 

 King, Jr. 



Disclosure: METHOD OF CONTROLLING 

 SEA LAMPREY 



Number: Application Serial Number 



340,573 



Date: March 27, 1964 



Use: Control of sea lamprey popula- 



tions using halogenated nitro- 

 salicylanilides, confined to a 

 class in which the nitro group 

 is substituted at either the 3 or 

 the 5 position on the aniline ring. 

 Controlled application of these 

 compounds should permit the ef- 

 fective eradication of larval lam- 

 preys in a river without signifi- 

 cant harm to other aquatic life. 

 John H. Howell and Everett L. 

 King, Jr. 



Inventors: 



Canada 



Disclosure: 



Number: 

 Date: 

 Assignee: 

 Use: 



METHOD FOR CONTROLLING 



PETROMYZON MARINUS 

 600,326 



Patented June 21, 1960 

 United States of America 

 Control of sea lamprey popula- 

 tions by the chemical treatment 

 of streams with 3-bromo-4-ni- 

 trophenol, a selectively toxic 

 agent. Controlled applications 

 permit destruction of all larval 

 lampreys in a watershed without 

 causing significant harm toother 

 aquatic life. 

 Inventors: Vernon C. Applegate and John H. 

 Howell 



Disclosure: METHOD FOR CONTROLLING 

 SEA LAMPREYS (PETRO- 

 MYZON MARINUS) 

 Number: 665,469 



Date: Patented June 25, 1963 



Assignee: United States of America 



Use: Control of lamprey populations 



by the chemical treatment of 

 streams with O -ethyl-S-penta- 

 chlorophenyl thiolcarbonate, a 

 selectively toxic agent. Con- 

 trolled applications permit 

 destruction of all larval lampreys 

 in a watershed without causing 

 significant harm to other aquatic 

 life. 

 Inventors: Vernon C. Applegate and John H. 

 Howell 



Disclosure: METHOD FOR CONTROLLING 



PETROMYZON MARINUS 

 Number: 683,165 



Date: Patented March 31, 1964 



Assignee: United States of America 



Use: Control of sea lamprey popula- 



tions by the chemical treatment 

 of streams with 2-bromo-4- 

 nitrophenol, 5-chloro-2-nitro- 

 phenol and their alkali metal 

 salts. Controlled applications 

 permit destruction of all larval 

 lampreys in a watershed without 

 causing significant harm toother 

 aquatic life. 

 Inventors: Vernon C. Applegate and John H. 

 Howell 



Patent applications in Canada corresponding 

 to the following United States applications have 

 been filed in the names of VernonC. Applegate 

 and John H. Howell as joint inventors: 



(1) METHOD FOR CONTROLLING SEA 



LAMPREYS WITH HALOGEN 



NITROPHENOLS 

 Application Serial Number 652,316 

 Filed April 11, 1957 (in the United 

 States of America) 



(2) METHOD FOR CONTROLLING 



PETROMYZON MARINUS 

 Application Serial Number 726,021 

 Filed April 2, 1958 (in the United 

 States of America) 



Patent applications in Canada corresponding 

 to the following United States applications 

 have been filed in the names of JohnH. Howell 

 and E. L. King, Jr., as joint inventors: 



(3) METHOD OF CONTROLLING SEA 



LAMPREY 

 Application Serial Number 336,079 

 Filed January 6, 1964 (in the United 

 States of America) 



(4) METHOD OF CONTROLLING SEA 

 LAMPREY 

 Application Serial Number 340,573 

 Filed March 27, 1964 (in the United 

 States of America) 



SCIENTIFIC STAFF, DECEMBER 31, 1964 



[C, Chemist; all others are 

 Fishery Biologists] 



Ann Arbor, Mich. 



James W. Moffett, Laboratory Director 

 Leo F. Erkkila, Assistant Laboratory Di. 

 rector. Sea Lamprey Research 



52 



