CONTENTS 



Page 



Introduction 1 



Fish protein concentrates 1 



Definition of biological procedures 2 



Objectives of program 2 



State of the art 2 



Literature survey 2 



Enzymatic hydrolysis of fish 2 



Acid/alkaline hydrolysis 4 



Fish fermentation 4 



Summary of NMFS work (generalized) 5 



In-house 5 



Bertullo process 5 



Relative activities of commercial enzymes 6 



Whole hake products 6 



Amino acid contents 8 



Alkaline bacterial proteases 8 



FPC solubilizations 8 



Replicate runs with selected enzymes 9 



NMFS contract research 10 



Columbia contract: fish fermentation 10 



Screening of microorganisms 10 



Additional studies 10 



Proteolytic microorganisms 10 



MIT contract: solubilization 11 



Alkaline solubilization 11 



Proteoljrtic enzymes 11 



Batch studies H 



Continuous reactor 12 



Summary of experimental results 12 



Summary of screening tests 12 



Autoljrtic activity red hake 13 



Effect of cooking on degree of digestion 14 



Autolysis of raw hake 15 



Microbiological aspects 15 



Yields, PER, compositions for various enzymes 16 



Yields 16 



Amino acid compositions 16 



Tryptophan concentration 16 



Histidine concentration 17 



Replicate runs, red hake 18 



Replicate runs, alewife 18 



Protein efficiency ratios (PER) 20 



Material balances 21 



Hydrolysis of red hake 21 



Hydrolysis of alewife 22 



^ Marin 3 'ChiogTc^.TTl- jrTryl 



LIBRARY 



DEC 1 2 1972 



Woods Hola, Mass. 



