The herring stickwater or press liquor used in Diet 8 contained about 

 25 per cent protein and 7 per cent oil, according to the manufacturer, 

 a British Columbia fish reduction plant. 



On the basis of an anemia check of over 500 fish in this group, 

 the fish fed Diet 5 (crab meal), Diet 7 (crab meal and viscera meal), 

 and Diet 9 (beef liver) were found to have no anemia. The lots fed 

 Diet 6, with viscera meal only, had an anemic tendency, and the lots 

 fed Diet 8, with herring s.tickvjater, had a pronounced anemia, 



'J-'he final weights and mortalities disclosed that the herring 

 stickwater in Diet 8 reduced the growth rate and increased the mor- 

 tality rate when compared with the control of this group. Diet 6, 

 This particular supplementation of stickwater at the 20 per cent level 

 in this diet was quite unsatisfactory especially since the lot went 

 anemic toward the end of the experiment. 



The beef liver supplement proved to be more promising than the 

 stickwater supplement since, although the survivals were no better 

 than in the group control, the final mean weight was significantly 

 greater than that produced by either the control or the stickwater 

 variant. 



The crab meal substitution for salmon viscera meal proved de- 

 cidedly beneficial. Additional comments on crab meal will be found 

 in the next section devoted exclusively to crab meal. 



Comparison of Crab Meal with Salmon Meals 



On the basis of work by other investigators in fish nutrition, 

 crab meal was included in several diets to determine its effect on 

 anemia and growth. 



The crab meal was derived from the total crab scrap of the blue 

 crab ( Callinectes sapidus ) and reduced to a dry powder in a conventional, 

 rotary flame-drier. 



The diet number and components of the crab meal diets were: Diet U 

 with crab meal at 20 per cent, hake and salmon viscera at UO per cent 

 each; Diet 5 with crab meal at 20 per cent, and hog liver and salmon 

 viscera at UO per cent each^ • Diet 7 with" crab meal and lU5° tunnel-dried 

 salmon viscera meal at 10 per cent each, and hog liver and salmon vis- 

 cera at UO per cent eachj Diet 11 with $ per cent each of crab meal and 

 flaiae-dried salmon offal meal added to a mixture of 20 per cent each of 

 beef liver, hog liver, hog spleen, and 30 per cent of salmon viscera. 



The purpose of Diet i; was to determine if anemia would develop in 

 this combination of hake, salmon viscera, and crab meal. In the 19 U8 



15 



