representing essentially complete agglutination 

 and 3+, 2+, and 1+ lessening degrees to or no 

 agglutination. 



Two general kinds of antibodies were 

 employed. Those found in "normal" bovine and 

 sheep serums, and those found in the serums 

 of individual rabbits previously injected with 

 the cells of one or another kind of fish. The 

 reactions of these serums with fresh tuna cells 

 were surveyed (Cf. table 7) preliminary to fur- 

 ther tests. This survey showed marked varia- 

 tions among the specificities and titers of the 

 serums, and indicated that antibodies capable 

 of reacting with fresh tuna cells are not uni- 

 versally present in rabbit serums at dilutions 

 of 1 in 32 or 1 in 50. Frozen whole bloods, 

 diluted 1 in 4, were used in the preparation of 

 the serums, and a variety of injection sched- 

 ules, all in use at one laboratory or another, 

 was followed. No general conclusions can be 

 reached as to which of these was the most suc- 

 cessful . As fresh cells of the species used for 

 immunization were usually not available at the 

 time of collection, cells of other species, 

 capable of cross -reacting with the serums, 

 were often used in preliminary titration. All 

 serums were kept frozen without the addition 

 of preservatives when not in use, some being 

 thawed and refrozen as much as a dozen times 

 without apparent change in titer and specificity. 

 Serums were readily transported to and from 

 the Hawaiian Islands in an insulated cloth picnic 

 bag containing dry ice. Characteristics of the 

 serums that were used the most extensively in 

 these studies are given below; additional prop- 

 erties are described where they specifically 

 pertain to the experiments described in this 

 paper . 



Anti yellowfin tuna 2 - immunization 

 with aliquots of whole blood of a 

 single yellowfin tuna ( Neothunnus 

 macropterus Temminck and Schlegel) 

 collected by P . O . F . I . at Canton Is - 

 land, June, 1951. 



Anti -oceanic skipjack - immunization 

 with aliquots of whole blood of a 

 single oceanic skipjack (Katsuwonus 



pelamis Linnaeus) collected by 

 P. O.F.I, at Christmas Island, 

 June, 1951. 



Anti albacore 10 - immunization with 

 aliquots of the whole bloods of four 

 albacore (Germo alalunga Gemlin) 

 collected by E . K . Holmberg off 

 the Oregon coast, July, 1952. 



Anti -albacore 14 - immunization with 

 aliquots of the whole blood of four 

 albacore collected as above. 



Anti-mackerel - immunization with 

 aliquots of the whole bloods of two 

 Pacific mackerel (Pneumatophorus 

 japonicus diego Ayres) collected 

 at Santa Barbara, June, 1951. 



Normal bovine serum - obtained through 

 the courtesy of Lucian Sprague and 

 Dr. Clyde Stormont . Sample 

 C78 -2582 -2779 -1/20/53. 



Normal sheep serum purchased from 

 the Cappel Laboratories, West 

 Chester, Penn. 



Human serum - commercial anti -A and 

 anti-B typing serums obtained from 

 the Hyland Laboratories, Los 

 Angeles, Calif. 



The fresh Hawaiian fish referred to in 

 this paper were obtained from several sources 

 during the author's stay at the Hawaii Marine 

 Laboratory. The single little tunny or kawaka- 

 wa ( Euthynnus yaito Kishinouye) was taken by 

 Lester Zufceran while surface trolling near the 

 entrance of Kaneohe Bay . The single yellowfin 

 or ahi ( Neothunnus macropterus TemmincK and 

 Schlegel) was taken by Georges Gilbert while 

 surface trolling near the entrance of Kaneohe 

 Bay, Oahu . The single wahoo or ono 

 ( Acanthocybium solandri Cuvier and Valen- 

 ciennes) was taken by Georges Gilbert about 

 one mile off Diamond Head, Oahu. The ocean- 

 ic skipjack or aku ( Katsuwonus pelamis 

 Linnaeus) were taken commercially by Harry 



