Mouse Number Amount of Inoculum Time of Inocula tion Reaction 



10 1 ml 6 % k9 P»m 1/ 



11 1 ml 6tU5 Pom T/ 



12 1 ml 6sl|6 p.m„ 2/ 



l/ Mice reacted with symptoms similar to those resulting from the 



injection of the unheated extract! however , they recovered completely 

 by 8s 00 a m the following morning „ 



2/ Mouse No 12 had symptoms similar to those resulting from the injection 

 of unheated extract and died at 9^10 a* the following morning » 



Ten grams of the raw flesh were fed each of three mice that had 

 been fasted for 2-U hours „ The mice showed no ill effects., No definite 

 conclusions regarding the nature of the poisoning agent can be made from 

 the above data 



Discussion 



An extensive review of the literature on fish poisoning discloses 

 that very little is definitely known regarding this phenomenon except the 

 symptomology, a few of the species of fish involved, and the localities 

 where the poisoning is preva lento Some investigators have placed into print 

 native folklore under the guise of scientific investigation Numerous 

 references are made as to the nature of the "toxic agent," seasonal incidence 

 of poisoning, and methods far distinguishing poisonous from non-poisonous 

 fish, without an iota of research data to support thenu It is hoped that 

 this paper will dispel some of the erroneous ideas, posed as scientific 

 facts, regarding fish poisoningo 



The present investigations have done little to enlarge our knowledge 

 of fish poisoningo It was found fish poisoning is not a seasonal phenomenon 



No progress concerning the cause or the causative agent of fish 

 poisoning can be made unless some actual research work is done» An active 

 research program is necessary to prove or to disprove the various theories 

 regarding fish poisoning. These investigations should consist of the 

 following studies % 



lo Studies of the life history and ecology of the various 

 species of fish responsible for poisoningo 



2o Feeding experiment using more suitable test animals 



3° Extensive bacterial and chemical tests on samples of 

 fish which are found to be toxic to laboratory animals 

 in order to try to find the causative agent of the disease 



U° Collection of epidemiological data on all so-called "fish 

 poisoning cases," to determine whether or not they are the 

 result of true "fish poisoning," food poisoning, or a 

 combination of the two 



L9 



