Each hatchery had its own cold-storage facilities for the storage 

 of the various constituents of the diets. However, since Winthrop and 

 Entiat hatcheries had limited coli-storage facilities, the majority of 

 their viscera was stored at the Leavenworth hatchery. Periodically, 

 limited amounts of the viscera were shipped to the Entiat and Winthrop 

 hatcheries. Except in special instances, no effort was made to segregate 

 the fish products used at any of these three hatcheries. Lots of viscera 

 at Leavenworth were treated as a common supply for the Entiat, Winthrop, 

 and Leavenworth hatcheries. Blocks from any one lot were used at each of 

 the three hatcheries. 



Little information is available concerning the species of salmon 

 from which the viscera was obtained. It is believe! that the majority 

 of the viscera fed at the Winthrop, Entiat, and Leavenworth hatcheries 

 in 1953 was from pink and sockeye salmon, and the eggs fed at the Cook 

 hatchery from chinook and chum salmon. 



The diet fed at the Leavenworth and Winthrop hatcheries consisted 

 of approximately 30 percent salmon viscera and 70 percent a mixture of 

 one or more of the following products: beef-liver, hog-liver, hog-spleen, 

 or beef lungs. The diet fed at the Oook hatchery did not include any 

 viscera, but rather a mixture of 30 percent salmon eggs and 70 percent 

 beef and hog products. The diet at the Issaquah hatchery included fish 

 viscera, fish carcasses, and beef-liver. Although all of the troughs of 

 fish were fed the same diet at each hatchery, not all of the troughs be- 

 came infected (table 1). 



There were two groups of sockeye fingerlings reared at the Entiat 

 hatchery in 1953. In the first group, 12 of the 11+ troughs were fed a 

 diet of beef-liver, salmon viscera, and additional fish products of hake 

 and arrowtoothed halibut in various proportions. The diet far the fish 

 in the two remaining troughs of this group consisted of pure beef-liver — 

 no fish products were incorporated. The viscera for the first group of 

 fingerlings were from a lot set aside early in the season in order that 

 a minimum variation be present in the viscera for the planned nutritional 

 experiments. All troughs in the first group of fish (fig. h, troughs 1A 

 through 13B) became infected with the exception of the two troughs which 

 were fed a pure beef-liver diet (fig. h, troughs la and lb) . 



The second group of sockeye fingerlings at the Entiat hatchery 

 (fig. Ii, troughs 31-ii5) received a diet similar to that fed at the Leaven- 

 worth and Winthrop hatcheries. The viscera fed to this group of finger- 

 lings were taken at random from the cold-storage facilities at Leavenworth; 

 no attempt was made to feed this group viscera from a designated lot. 

 Only 2 of 12 troughs of fingerlings became infected in this group. 



13 



