CONTENTS 



Page 

 Part I: Response of tuna to chemical stimuli 1 



Fishingc 2 



Establishment of tunas in captivity 3 



Concrete taink 3 



Pond Noo 5 4 



Feeding 5 



Methods and procedures in testing , 7 



Concrete tank 7 



Pond Noo 5 11 



The response 13 



Problems in testing 16 



Weather 16 



Power failure 17 



Erratic behavior of the fish 17 



Measurement of response 18 



Variation in response. 24 



Source and preparation of test substauices 27 



Materials c 27 



Prepeuration 28 



Response to simple extracts of tuna and other fish 30 



Flesh extracts of tuna 30 



Flesh extracts of other fish 32 



