Bigeye fleshj 1168 go alcohol extract 



1 



Filtrate 



Lead acetate added; filtered 



Filtrate 



Treated with sodium 

 chloride 



r 



i_ 



No„ Z66 

 XXX 



Filtrate 5 

 boiled 



No„ 267 

 (X) 



Passed through strong cation 

 exchanger 



Filtrate 



About pH 3 Adjusted to Passed through 

 I pH 5 to 7 strong anion 



exchanger 



Noo 268 Noo 269 j j 



Filtrate Eluate 



NOo 261 NOo IhZ 



Figure 12o ■= --Fractionation of an alcohol extract of bigeye flesh; 



procedure and results in experiments Nob- 261 j, 262, 

 266 to 269p 272„ and 277o 



49 



