Bigeye fleshy ZOOO go , 1st alcohol extract 

 filtrate 



Lead acetate added; filtered 



Sodium chloride added to filtrate; filtered 



Filtrate 



Heated on Through weak 

 water bath cation exchange 

 I column 



No, 273 



Filtrate 



Through strong cation 

 exchange colunnn 



Filtrate 



XXX 



NOo Z78 

 X 



NOo 274 Through strong Through weak 

 anion e><change anion exchange 

 column column 



Eluate 



j 



No„ 275 



Eluate 



! 



Noo 276 



Figure 13o --Fractionation of a first alcohol extract of bigeye 

 flesh; procedure and results in experiments 

 NoSo 273 to 276„ and 278o 



51 



