Bigeye flesh, 2000 g. , 2nd alcohol extract, 

 filtrate 



Lead acetate added, filtered 



Filtrate acidified with hydrochloric acid, 

 filtered 



Filtrate 



No. 280 Evaporated 

 to dryness 

 XX I 



Dissolved in 

 absolute 

 alcohol 



No. 281 



XX 



Adjusted to pH 9 



Passed through weak anion 

 exchange column 



Eluate 



No'. 282 



Filtrate 



No, 283 



XX 



Figure 14. --Fractionation of a second alcohol extract of bigeye 

 flesh: procedure and results in experinnents 

 Nos. 280 to 283. 



52 



