Bigeye flesh, 2000 g. , 3rd alcohol extract, 

 filtrate 



Lead acetate added, filtered 



Filtrate acidified with hydrochloric acid, 

 filtered 



Filtrate adjusted to pH 7 



Passed through strong cation exchange 

 column 



Eluate 



No 



287 



Filtrate 



pH 2 pH 



XX 



No. 285 No, 286 

 X 



Figure 15. --Fractionation of a third alcohol extract of bigeye 

 flesh: procedure and results in experiments 

 Noso 285 to 287. 



54 



