Table 2. — Serological differences between races of 

 Fraser River sockeye salmon. 



* Significantly different at the 95 percent level. 



both tributary areas on the Fraser River 

 in British Columbia (table 1). The three 

 samples tested from the Adams River race 

 removed all or nearly all of the antibodies 

 present in the serum. The three samples 

 from the Cultus Lake race each removed all 

 of the antibodies specific for their anti- 

 gens, but left cintibodies which would react 

 with cintigens present on the cells of other 

 individual tested. Thus, three reagents 

 of different specificites were pro- 

 duced. 



Chinook salmon from the Columbia 

 River, resulted in the production of 

 reagents of four different specifici- 

 ties. The results indicate a charac- 

 teristic is present in number one 

 which is absent from the other three. 

 Superimposed on this, there appears 

 to be a kind of subtype difference 

 between 2, 3, and 4 which is shared 

 with 1. 



Evidence for blood groups in 

 chum salmon (0. keta ) is presented in 

 tables 4 and 5. The experiments out- 

 lined in these tables were performed 

 with samples from the Samish River in 

 Washington State. Evidence for the 

 presence of a considerable amount of 

 antigenic heterogeneity in this species and 

 this race is provided by the results pre- 

 sented in table 4, since reagents of four 

 different specificities were obtained by 

 five different absorptions. 



Five patterns of reactivity were dis- 

 played by the samples tested; numbers 1, 6, 

 8, and 9 reacted with the sera absorbed by 

 2, 4, and 5; numbers 2 and 7 reacted with 



These results as well as addi- 

 tional tests made on sajnples col- 

 lected from these areas in 1956 with 

 the above absorbed sera are combined 

 in table 2. Statistically signifi- 

 Ccuit differences in the proportion 

 of individuals reacting positively 

 with these reagents are apparent for 

 the two populations. 



Some of the antisalmon red 

 blood cell sera, prepared in rabbits 

 and chickens, were useful in demon- 

 strating blood group differences in 

 species of salmon other than the one 

 used for immunization. This is 

 somewhat analogous to the demonstra- 

 tion of the Rh blood groups in humans 

 by Landsteiner and Weiner (1941), 

 through the use of antirhesus monkey 

 red blood cell sera. 



The evidence for blood groups 

 in Chinook or king salmon (0. tsha- 

 wytscha ) is presented in table 3. 

 Absorption of serum R13, an anti- 

 sockeye salmon red blood cell serum, 

 by the cells of four different 



Table 3. — Demonstration of individual antigenic differences 

 in Chinook salmon erythrocytes using rabbit antisockeye 

 salmon serum (Serum R13 absorbed and cross-tested with 

 four individual samples of Chinook erythrocytes). 



(a) This absorbed serum tested against the cells of an 

 additional 12 chinook salmon, none of which reacted. 

 Positive reactions were obtained with the cells of 

 12 different sockeye salmon. 



(b) This absorbed serum tested against the cells of 24 

 additional chinook salmon. Definite reactions 

 occurred with 16 of them. 



