Table 4. 



the sera resulting from absorption 

 by 1 and 5; number 4 reacted with 

 the sera resulting from absorption 

 with 1, 3, emd 5; number 3 reacted 

 with sera from absorption with num- 

 bers 1, 2, 4, and 5; and number 5 

 reacted with the sera from absorp- 

 tion with numbers 2 and 4. The re- 

 sults obtained with individuals 2, 4, 

 and 7 demonstrate some of the com- 

 plexities involved in the antigenic 

 differences between individuals of a 

 single species. All gave the same 

 reaction with the reagents prepared, 

 except for the weak reaction of the 

 cells of individual 4 with the re- 

 agent prepared by absorption with 

 the cells of individual 3. Consist- 

 ent with this is the fact that reagents of 

 identical specificity were prepared by 

 absorption with cells of individuals 2 and 

 4. In contrast, absorption by cells of 

 individual 7 removed the antibodies reactive 

 with the cells of all individuals tested, 

 but the cells of individual 7 failed to 

 react detectably in agglutination tests with 

 reagents prepared by cibsorption with cells 

 of individuals 2, 3, or 4. Such apparent 

 deviations from the principles upon which 

 absorption analyses are generally assumed 

 to rest have been associated, in other spe- 

 cies, with the multiple-allelic control of 

 sets of related but nonidentical specifici- 

 ties. The large amounts of cells required 

 to absorb the antibodies present in this 

 serum which would agglutinate the cells of 

 all members of the species, precluded 

 further investigation of this system. 



-Demonstration of blood types in chum salmon 

 using chicken antichinook salmon serum 



(Serum 202 absorbed and tested with 

 cells of individual chum salmon) 



Test cell sample numbers 

 3 4 5 6 7 



+ ++00 + 



++ ++++ ++ + 

 + 000 



++ +++ ++ 

 ++ +++ 



+++ +++ 



++ ++++ 



The results presented in table 5 are 

 less complicated with apparently a single 

 difference detected between chum salmon 

 sample 18 and the others tested. Superim- 

 posed on this however are rather marked 

 differences in the strength of reactivity 

 of the other cells tested which may indi- 

 cate additional antigenic heterogeneity. 



Evidence for the existence of blood 

 group differences in pink salmon (0. gor - 

 buscha ) is presented in table 6. A single 

 difference was noted which appeared to be 

 correlated with the area of origin of the 

 samples tested. However, positive state- 

 ments about area differences must await 

 analysis of a larger number of samples. 



In addition to rabbit 2ind chicken 

 immunizations, extensive isoimmunizations 



Table 5. — Demonstration of blood types in chum salmon 

 using chicken antisockeye salmon sera. 



Sera 



11 



12 



13 



Test cell sample number 

 14 15 16 17 18 19 20 21 22 23 24 



++ ++++ ++++ ++++ 















n- ++++ +++ +++ 















+++ + +++ +++ ++++ +++ 















