Five fluorescent pigments provided suitable 

 secondary marks which could be used with 

 fast green as a combination nnark in experi- 

 ments designed to estimate rates of growth 

 and mortality. It was possible to localize the 



fluorescent material in two areas of the 

 shrimp's body, thereby increasing the nunnber 

 of connbination nnarks for shrimp. These 

 marks were permanent and did not affect 

 survival. 



ACKNOWLEDGMENT 



Acknowledgment is extended to Charles 

 Knight and Kenneth Osborn for advice and 

 assistance rendered during this study. Clinton 

 E. Atkinson, BCF Biological Laboratory, 

 Seattle, Wash., furnished a tattooing machine. 

 Fred C. June, BSFW, Pierre, S.D., and Thonnas 



J. Costello and Donald Allen, BCF, Miami, 

 Fla., provided advice on fluorescent pigments 

 and biological stains. Switzer Brothers, Inc., 

 Cleveland, Ohio, and General Dye stuff Com- 

 pany, New York, N.Y., furnished samples of 

 fluorescent pigments. 



LITERATURE CITED 



COSTELLO, THOMAS J. 



1964. Field techniques for staining- recap- 

 ture experiments with commercial 

 shrimp. U.S. Fish Wild. Serv., Spec. 

 Sci. Rep. --Fish. 484, 13 p. 

 DAWSON, C. E. 



1957. Studies on the nnarking of commercial 

 shrimp with biological stains. U.S. Fish 



Wild. Serv., Spec. Sci. Rep. --Fish. 231 , 

 24 p. 

 DUNSTAN, WILLIAM A., and WALLACE E. 

 BOS TICK. 

 1956. New tattooing devices for marking 

 juvenile salmon. Wash. Dep. Fish., 

 Fish. Res. Pap. 1 (4):70-79. 



MS. #1454 



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