CONTENTS 



Page 



Introduction . .,.0 ..... oo = .o. o. ..o ... t » -o .-.<.« o .<,.... o . ,^ ...,. o o o.. . 1 



Procedures for testing sterility and enumerating organisms ....... 2 



Sterility testing media ..................................... 2 



Inoculation and incubation procedures .,..,......,.........,, 4 



Miscellaneous checks for sterility . „,o ...,.<,........,.,.... . 5 



Procedure for enumerating bacteria .......................... 5 



Procedure for enximerating dinof lagellates ...„,.„„„,.. o ..... . 6 



Experiments with unialgal cultures of C-ymncdinium brevis and 



other dinof lagellates ...,.....,..,..,..,..,„..,.....,....,..... 6 



Experiment 1. A simple test of the toxicity of Gynnodinium 



Experiments 2 and 3 . Comparison of effects of Gymnodinium 



brevis and Gymnodinium splendens cultures ................ 7 



Experiment 4. Effects of unialgal Gymnodinium brevis 



cultures and the associated bacterial flora ...,.„,,....,. 8 



Experiment 5 . The effects of unialgal Gymnodinium brevis 

 cultures, the associated bacterial flora, and unialgal 

 Prorocentrum sp . cultures .. c ....... ...... ... o .... ,0,.. o, . 10 



Experiment 6. Comparison of toxicity of unialgal cultures 

 of Gymnodinium brevis and Prorocentrum sp,, and effects 

 of heating and filtration on toxicity ..................... 12 



Experiment 7. Comparison of toxicity of imialgal 



Gymnodinium brevis , Prorocentrimi sp,, and G:ymnodini''jm 



sp,, and effects of filtration on toxicity .............. 16 



Discussion of results of experiments with unialgal cultures . 17 



Experiments with bacteria-free cultures of Gymnodinium brevis . , . , 20 



Experiment 8. Effects of unialgal and bacteria-free 



G?/mnodinium brevis cultures .,.„,,„..„,...„ ......... o ..., . 20 



Experiment 9. Effects of bacteria-free and unialgal 



Gymnodinium brevis cultures, and effects of filtration 



on toxicity .....-•..„....,,,...„„.„.,....„.,.,»... o ....... . 2^^ 



Experiment 9a. Supplementary toxicity tests of some test 



materials previously used in Experiment 9 .,...,.,...,..., 31 



Discussion of results of experiments with bacteria-free 



cultures ................................................. 33 



