the following equations: 

 Chlorophyll types: 



Ca = 15.6D665 " 2.00545 - 0. 80530 

 (in mg/1 of extract) 



Cb = 25.40645 - 4.40^60 " 10.30630 

 (in mg/1) 



Cc = 10.9D630 - 12.50660 " 28.70665 

 (in MSPUA)--^ 



where C^ = concentration of chloro- 

 phyll a; C{3 = concentration of chlorophyll 

 b; Cc = concentration of chlorophyll c. 



jet of distilled water before removing caps. 



2. Pipette 3-milliliter samples in 

 duplicate into 50-milliliter Erlenmeyer 

 flasks using 30-milliliter beakers as 

 covers . 



3. Add reagent to groups of 12 sam- 

 ples (6 duplicates) as follows: 



a. At time 0, add 5 ml . of 

 reagent to sample I from the first 

 automatic acid burette. 



b. At time 30 seconds, add 5 ml. 

 of reagent to sample II from second 

 automatic acid burette. 



Carotenoid types: 



Ores, 510 = D5IO " 0.0026Ca - 0.0035Cb 



- 0.0021C(- 



Dres, 480 = D480 - 0.0019Ca - 0.0136Cb 



- 0.0054Cc 



<^astacin = 2(4.45Dj.gg_ 510-Dj.gg^ 480) 

 (in MSPU/L) 



•-^non-astacin ^ "^ -^ ^ '^res, 480 " 



1 -490^^0 on) (in MSPUA) 

 res, 51U 



In all above equations refers to the 

 optical density reading at the given wave 

 length. 



"Carbohydrates" 



We used the method of Zein-Eldin and 

 May (1958) based upon the method of J . G. 

 Erdman and A. B. Little (unpublished manu- 

 script) for determining the arabinose equi- 

 valent of "carbohydrate-like" material in 

 sea water. 



The determination is carried out as 

 follows: (Rinse all glassware used in pre- 

 paring and transferring the reagent in 

 c. p. H2S0^.) 



1. Thaw frozen samples with the aid 

 of a fan. Clean cap-glass junctions with a 



c. At 60 seconds, add an addi- 

 tional 22 ml. to sample I from the 

 first burette (27 ml. total reagent 

 added) . 



d. At 90 seconds, add an addi- 

 tional 22 ml. of reagent to sample II 

 from the second burette. 



e. Pump up both burettes and 

 continue until acid has been added to 

 all 12 samples. 



4. Place the samples in a water bath 

 at 70° C. for 30 minutes. 



5. Place the samples in the refri- 

 gerator and cool to room temperature. 



6. Read in the Fisher electrophoto- 

 meter using the G. A. B. 1^ 560 mu inter- 

 ference filter in the position or, if 

 unavailable, a 525 B filter in the B posi- 

 tion. Read against distilled water. 



7. Treat triple-distilled water 

 blanks and arabinose standards of 1, 2, 3, 

 5, and 10 mg, per liter similarly. 



N-ethyl carbazole reagent: 



Crude N-ethyl carbazole is re- 

 crystallized from an ethanol solution 

 and precipitated with triple-distilled 

 water until pure white, needle-shaped, 



kj MSPU/L = one- thousandth of a specified 

 pigment unit (SPU). 



^ Obtained from Photovolt Corporation, 

 New York. 



11 



