92 



lysis by HCl might also been explained as caused by the carbo- 

 hydrates originating from the nucleins?). 



The same result, however, was obtained also for the gemmules 

 after I had almost entirely freed their rubbed material of (coarser) 

 solid particles (among others of algae and oil-droplets) by centri- 

 fuging and extracting with ether. Moreover, the living cells of 

 the gemmules considerably swell in water, so apparently possess 

 a strongly hypertonic cell-fluid, which corresponds very well with 

 the presence of dissolved sugars. 



So we may conclude, that a polysaccharide is present in the 

 sponge-tissue also beyond the (coarser) solid parts of the cells; 

 we now can freely explain the above mentioned small globules 

 stainable brown by I as consisting of the polysaccharide in question. 



13. As appears from Table 14, these small globules are present 

 in about the same number in amoebocytes of a green sponge in 

 light, so in amoebocytes filled up with green algae, as in those 

 of a colourless sponge from darkness, so with but few green 

 algae; it may even be, that these globules are somewhat more 

 numerous in the second than in the first cells. From this we 

 may conclude again, that export of carbohydrates from the green 

 algae probably does not take place at all. For if it were the case, 

 these carbohydrates in solution — in 11 namely it appeared, that 

 these will be present in the algae — , which would then evi- 

 dently be deposited in the sponge tissue partly in the form of 

 reserve food (the little globules), had at any rate to be much 

 more numerous in the green sponge from light than in the colour- 

 less one from darkness, while exactly the reverse proved to be 

 the case. 



Perhaps one might be inclined to explain this last fact, just 

 as in 5 for the oildroplets, as being caused by a larger con- 

 sumption of the carbohydrates in a green sponge in light. I then 

 can give exactly the same answer, I have given already (in 5) 

 for the oildroplets, and which I am not going to repeat. 



As we saw that the little globules, which can be stained by 

 I, in the amoebocytes of a colourless sponge from darkness are 

 probably even somewhat more numerous than in those of green 



