213 



I sliould inention that the colours of I^, II^ and IIIj were absolutely 

 equal at the beginning of the experiment; this was also the case in 

 I3 — I,,, II3, II4, III3 and III j as well as in I,., — 1,5, II-, 11^, III5 and 

 Illg. The experiments I3 etc, II3 etc. and III3 etc. contained other 

 ,,enzyme" and ,,emulsion" than the preceding ones. 



In the first place we see from the table, that the ,, blind" experi- 

 mental series II and III lose indeed more or less their red colour. 

 Consequently, acids are set free in these. In II one might ascribe 

 this to liydrolysis due to tlie lipase, for this mixture must have also 

 contained some (few) oildrops. But in III no lipase can have been 

 present at all; nevertheless the fat is hydrolyzed — probably by means 

 of the alkali — (or the red colour diminishes by the entrance of COj 

 from the air). 



The expei'iments of series I, liowever, prove to lose their red colour 

 sooner than those of II and III. One might be inclined to explain 

 this by tlie combined inüuences, which were acting separately in II 

 and III. But that would not be exact, as is shown in the second and 

 in the last group of experiments. Still another factor must have been 

 acting. That must be the hydrolysis of the fat by the lipase. But 

 this lipase does not seem to be very active here; although it is ditfi- 

 cult to give a decision. 



Table 14. Th^ Huinher of oildrops mid of' glohules, tolticli eau he 

 stained (hrovm) hy 7, present in choanocytes in comparison to that 

 in anwehocytes^ in green and coJourless Spongillidae taken from 

 ivater of tlte lake, from water of the canal and from that of the 

 conduit ; microscopically examined by means of ravel preparations 

 (p. 12 — 15) of sponge tissue. 



I examined green Spongillae (s.) and Ephydatiae (e.) from light and 

 colourless ones from darkness. The (average) number of oildrops 

 (oildr.) and of globules which can be stained by I (glob. I) wasalways 

 stated in the same volume of amoebocytes (sub 1) and of choanocytes 

 (sub 2). As for the meaning of I — XII, see pag. 14. In the column 

 ,,remarks" is mentioned: the. species of the sponge; the month of 

 the examination; whether the sponges, after their capture from the 

 lake or the canal, had been in water from the conduit (aq.) before 

 they were examined, and for how many time; and whether sponge- 

 reduction had occurred. 



All experiments are mentioned. As for the discussion, see pag. 90 — 93. 



