3. Some extracts contained specific phyto- 

 precipitins which reacted with components in 

 fish sera when tested by the double diffusion 

 technique of Ouchterlony. 



4. The results of this research indicate 

 that plant extracts may be usefully applied as 

 haemagglutinins or serum precipitins in study- 

 ing fish populations. 



ACKNOWLEDGMENTS 



The University of Washington Arboretum 

 staff, particularly Joseph Witt, furnished many 

 of the seed and bark samples used in this 

 study and assisted in identifying numerous 

 legume species. Warren Ames and Melvyn 

 Mosher performed many of the agglutination 

 tests of this study. 



MAKELA, OLAVI. 



1957. Studies in hemagglutinins of Legu- 

 minosae seeds. Helsinki, Annales Medi- 

 cinae Experimentalis et Biologiae 

 Fenniae: Supplementum No. 35, 133 p. 



MORGAN, W. T. J., and WINIFRED M. WAT- 

 KINS. 

 1953. The inhibition of the haemaglutinins 

 in plant seeds by human blood group 

 substances and simple sugars. British 

 Journal of Experimental Pathology, vol. 

 34, no. 10, p. 94-103. 



OUCHTERLONY, ORJAN. 



1958. Diffusion-in-gel methods for immu- 

 nological analysis. Paul Kallos, Prog- 

 ress in Allergy, vol. 5, p. 1-78. 

 S. Karger, New York. 



LITERATURE CITED 



BIRD, G. W. G. 



1951. Specific agglutinating activity for 

 human red blood corpuscles in extracts 

 of Dolichos bifloms. Current Science (In- 

 dia), vol. 20, p. 298. 



1952. Relationship of the blood sub-groups 

 Ai, A2 and AiB, A2B to haemagglutinins 

 present in the seeds of Dolichos biflorus. 

 Nature, vol. 170, no. 4329, Oct.. 18, 

 1952, p. 674. 



1961. Paradoxical findings in Ouchterlony 

 tests. Experientia, vol. 17, fasc. 9, 

 Sept. 15, 1961, p. 408. 



BOYD, WILLIAM C, and ROSE M. REGUERA. 



1949. Hemagglutinating substances for 



human cells in various plants. Journal 



of Immunology, vol. 62, no. 3, p. 333- 



339. 



BOYD, WILLIAM C, and ELIZABETH SHAP- 



LEIGH. 



1954. Antigenic relations of blood group 



antigens as suggested by tests with 



lectins. Journal of Immunology, vol. 73, 



no. 4, p. 226-331. 



RENKONEN, K. O. 



1948. Studies on hemogglutinins present in 

 seeds of some representatives of the 

 family Leguminoseae. Helsinki, Annales 

 Medicinae Experimentalis et Biologiae 

 Fenniae, vol. 26, p. 66-72. 



RIDGWAY, G. J., J. E. GUSHING, and G. L. 

 DURALL. 

 1958. Serological differentiation of popula- 

 tions of sockeye salmon, Oncorhynchus 

 nerka. U.S. Fish and Wildlife Service, 

 Special Scientific Report--Fisheries 

 No, 257, 9 p. 



RIDGWAY, GEORGE J., and GEORGE W. 

 KLONTZ. 

 1960. Blood types in Pacific salmon. U.S. 

 Fish and Wildlife Service, Special Scien- 

 tific Report — Fisheries No, 324, 9 p. 



RIDGWAY, GEORGE J., GEORGE W. KLONTZ, 

 and CHARLES MATSUMOTO. 

 1962. Intraspecific differences in serum 

 antigens of red salmon demonstrated by 

 immunochemical methods. International 

 North Pacific Fisheries Commission, 

 Bulletin No, 8, p. 1-13. 



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