SUMMARY 



In order to find a method of uniformly 

 infecting large numbers of eastern brook trout, 

 to determine how the disease might be trans- 

 mitted in the hatchery, to compare two strains 

 of the bacterium, and to observe the course of 

 infection, transmission trials were carried out 

 for 102 days in water at 12.5° C. Infection of 

 eastern brook trout with kidney disease was not 

 possible by addition of the bacterium to the 

 fish's diet alone or with glass shards or with 

 bile salts. Infection was accomplished by man- 

 ual abrasion and to a lesser degree by auto- 

 abrasion in the presence of the kidney disease 

 bacterium . The eastern strain of the bacterium 

 infected about one-third of the self -abraded 

 group and about two -thirds of the manually 

 abraded group. Few infections were established 

 by either method with the western strain of the 

 bacterium . All infections developed slowly and 

 were chronic. 



Earp, Brian J., C. H. Ellis, and E. J. Ordal 

 1953. Kidney disease in young saln-on. 



Washington Department of Fisheries, 

 Spec. Rep., Ser. 1, 74, illus. 



Ordal, ErUngJ., and B.J. Earp 



1956. Cultivation and transmission of 



etiological agent of kidney disease io 

 salmonid fishes. Proc. Soc. Exp. 

 Biol, and Med., 92., 85-88, illus. 



Rucker, Robert R., W.J. Whipple, J. R. 

 Parvin,andC. A. Evans 



1953. A Contagious disease of salmon 

 possibly of virus origin. Fishery 

 Bulletin 76, U.S. Fish and Wildlife 

 Service, 54: 35-46, illus. 



Snieszko, Stanislas F. 



1954. Therapy of bacterial fish diseases. 

 Trans. Am. Fish Soc, 83(1953): 

 313-330. 



In an experiment intended to determine 

 the effects of temperature on the disease, fish 

 held at 7° C. and at 12.5° C. were given re- 

 peated puncture-type inoculations of the bacter- 

 ium . One strain of the bacterium produced 100 

 percent mortality at both temperatures, but it 

 was slightly more virulent at 7° C. The other 

 strain was less virulent, but its effect was also 

 more pronounced at the lower temperature. Re- 

 covery or failure to infect systemically occurred 

 at both temperatures with this bacterium . 



LITERATURE CITED 



Belding, David L., and B. Merrill 



1935. A preliminary report upon a 



hatchery disease of the Salmonidae . 

 Trans. Am. Fish. Soc. 65: 76-84. 



Deuel, C. R., D. C. Haskell, D. R. Brockway, 

 and C. R. Kingsbury 

 1952 . The New York State Fish Hatch- 



ery Feeding Chart. Fisheries 

 Research Bulletin 3, 3rd Ed., 59, 

 New York State Conservation Depart- 

 ment, Albany. 



Snieszko, S. F., and P. J. Griffin 



1955. Kidney disease in brook trout 



and its treatment. Progressive Fish - 

 Culturist, 17: 3-13, illus. 



Tunison, A. V., A. M. Phillips, Jr., C. M. 

 McCay, C. R. Mitchell, and E. O. 

 Rodgers 

 1939. The nutrition of trout. Cort- 



land Hatchery Report 8, New York 

 State Conservation Department, 33, 

 illus. 



Wood, James W., and J. Wallis 



1955. Kidney disease in adult chinook 



salmon and its transmission by feed- 

 ing to young Chinook salmon. 

 Research Briefs, Fish Commission 

 of Oregon, 6: 2, 32-40. 



INT -DUP SEC, WASH.. DC. 



